[Histonet] Antigenicity

[Rene J Buesa]

Patricia:
  Not at all! Antigenicity (due to antigen oxidation) is slowly lost when the control slides are stored for more than 4 weeks, although not all antigens oxidize at the same rate, some being more "resilient" than others.
  Some experiments have been done about how to prevent this "fading" and they go from storing the contols at 20ºC, or in a nitrogen atmosphere, or covering them with paraffin. I did use to put them in a container with mineral oil which isolated them from the air (although is somewhat messy and will prevent you to put the case section along with the control in the same slide).
  Hope this will help you!
  René J.

Patricia Karlisch <pkarlisch <@t> psu.edu> wrote:
  Hello all,
I have a technical question. Do any of you pre-cut your control
slides in larger batches? It used to be that the control slides would
lose their antigenicity if stored for long periods of time (several
months) in a slide file as opposed to cutting the blocks as needed. 
Have the HIER techniques evolved to the point that this is no longer
true? 
Thank you in advance. 
Pat

Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch <@t> psu.edu

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