[Histonet] Casein Question in IHC

Hermina Borgerink hborgeri <@t> wfubmc.edu
Wed Nov 29 10:09:43 CST 2006

Hi Lei,

I routinely use 0.5% casein in Tris buffer for all my antibody diluents
and washes.  After adding the appropriate number of grams to the buffer,
I leave the solution stirring overnight with the aid of a stir plate and
stir bar. Although the solution continues to have a cloudy aspect the
following morning, all the casein has actually gone into solution.
Ref.: Casein Reduces Nonspecific Background Staining in Immunolabeling
Techniques by David E. Tacha et al J. Histotechnology, 1992 15: 2, p.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lei Quan
Sent: Wednesday, November 29, 2006 10:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Casein Question in IHC

I had a problem to resolve casein in either PBS or Water when using it
for blocking apecific binding. Does anyone have the same problem? Can I
use BSA instead or can I heat a bit to help it resolve? Thanks!
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