[Histonet] RE: Histonet Digest, Vol 36, Issue 34

Wed Nov 29 10:03:26 CST 2006

Please check references when forwarding information, such as the Microsoft
scam below.

http://www.snopes.com/inboxer/nothing/billgate.asp

Joanne Malinowski
Histology Testing Service
Cambrex Bio Science Walkersville, Inc.
8830 Biggs Ford Road
Walkersville, MD 21793
(301) 898-7025 x 2244
Fax (301) 845-4153
e-mail: joanne.malinowski <@t> cambrex.com

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, November 29, 2006 9:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 36, Issue 34

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Today's Topics:

   1. Lectins and epithelial cross-reactivity (Milne, Katy)
   2. FULL- TIME OPENING FOR HT (Galiotto, Laura)
   3. FW: FULL- TIME OPENING FOR HT (Galiotto, Laura)
   4. Histotech Fellowship Opportunity (Arzt, Jonathan)
   5. Job opening at PhenoPath Laboratories (Patti Loykasek)
   6. flourescent microscope (Melody Thiessen)
   7. Cytochrome P450 antibody staining (Mark Elliott)
   8. RE: Richard Allan (connie grubaugh)
   9. re: macrophages/microglia in FFPE tissue (Carl Hobbs)
  10. RE: automated immuno stainer (Patsy Ruegg)
  11. RE: automated immuno stainer (Weems, Joyce)
  12. Missouri Society for Histotechnologists rewards
      (Rey, Jean-Philippe)
  13. SEEKING PAUL HASSEMER (Comcast Mail)
  14. Room temperature (donna rossi)
  15. Thank you all for your feedback (Heather Renko)
  16. weils stain for myelin (stevenk)
  17. Re: weils stain for myelin (Bryan Llewellyn)
  18. Orcein stain for fat in paraffin sections (Megan Clarke)
  19. Anti GFP on cryosections (Adi Sabag)
  20. Von Kossa stain (Brunella Spaggiari)
  21. FW: controls (Webb, Dorothy L)
  22. Fwd: Fwd: FW: READ!!!!!!! (Coleman Manufacturing)
  23. Don't fall for the Hoax in a just posted histonet (Lester Raff)
  24. RE: Don't fall for the Hoax in a just posted histonet
      (Dawson, Glen)
  25. Re: Fwd: Fwd: FW: READ!!!!!!! (Anne C Lewin)

----------------------------------------------------------------------

Message: 1
Date: Tue, 28 Nov 2006 10:14:49 -0800
From: "Milne, Katy" <kmilne <@t> bccancer.bc.ca>
Subject: [Histonet] Lectins and epithelial cross-reactivity
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<07979E76B0869D4E8C9FE4AA9FC065780240D180 <@t> srvex03.phsabc.ehcnet.ca>
Content-Type: text/plain;	charset="us-ascii"

Hi all, 

I was wondering, speaking of lectins, if anyone has experience using a
biotinylated lectin in FFPE mouse tumor tissue (not following perfusion,
just routine sectioning) to look at angiogenesis within the tumor.  I
understand that there could be some cross-reactivity to the epithelial
cells which for us would be a bit of a problem as our tumors are pretty
solid.  Has anyone seen the problem and how bad is it?  I'm getting
tired of trying to get CD31 and CD34 to behave nicely in our FFPE tumors
but I need to be able to see the tumor vasculature.

Thanks,

Katy Milne

Deeley Reseach Centre,

BC Cancer Agency,

Victoria BC

------------------------------

Message: 2
Date: Tue, 28 Nov 2006 12:37:54 -0600
From: "Galiotto, Laura" <LGaliotto <@t> nch.org>
Subject: [Histonet] FULL- TIME OPENING FOR HT
To: <HISTONET <@t> LISTS.UTSOUTHWESTERN.EDU>
Message-ID:
	<270614B321ACB44D8C1D91F4F921FDC304B1618F <@t> NCH01EX02.nch.org>
Content-Type: text/plain;	charset="iso-8859-1"

Hello Everyone
   I have a full-time opening for a Histology Technician, must be ASCP
certified. Tues-Sat 3rd shift some flexibility in start time. The hospital
is wonderful place to work and excellent benefits.
Those that are interested please apply on-line at www.NCH.org

Thank you 
Laura 
Histology facilitator
Northwest Community Hospital 
Arlington Heights, IL

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the message and any accompanying documents.  Thank you. 

------------------------------

Message: 3
Date: Tue, 28 Nov 2006 12:42:01 -0600
From: "Galiotto, Laura" <LGaliotto <@t> nch.org>
Subject: [Histonet] FW: FULL- TIME OPENING FOR HT
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<270614B321ACB44D8C1D91F4F921FDC304B16191 <@t> NCH01EX02.nch.org>
Content-Type: text/plain;	charset="iso-8859-1"

>  -----Original Message-----
> From: 	Galiotto, Laura  
> Sent:	Tuesday, November 28, 2006 12:38 PM
> To:	'HISTONET <@t> LISTS.UTSOUTHWESTERN.EDU'
> Subject:	FULL- TIME OPENING FOR HT
> 
> Hello Everyone
>    I have a full-time opening for a Histology Technician, must be ASCP
certified. Tues-Sat 3rd shift some flexibility in start time. The hospital
is wonderful place to work and excellent benefits.
> Those that are interested please apply on-line at www.NCH.org
> 
> Thank you 
> Laura 
> Histology facilitator
> Northwest Community Hospital 
> Arlington Heights, IL
> 

******************* PLEASE NOTE *******************
This E-Mail/telefax message and any documents accompanying this transmission
may contain information that is privileged, confidential, and/or exempt from
disclosure under applicable law and is intended solely for the addressee(s)
named above.  If you are not the intended addressee/recipient, you are
hereby notified that any use of, disclosure, copying, distribution, or
reliance on the contents of this E-Mail/telefax information is strictly
prohibited and may result in legal action against you.  Please reply to the
sender advising of the error in transmission and immediately delete/destroy
the message and any accompanying documents.  Thank you. 

------------------------------

Message: 4
Date: Tue, 28 Nov 2006 14:08:11 -0500
From: "Arzt, Jonathan" <Jonathan.Arzt <@t> ARS.USDA.GOV>
Subject: [Histonet] Histotech Fellowship Opportunity
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<6B4AF69268EFAE4C80A786BA99F7A667B41F84 <@t> MD-MAIL-01.ARSNET.ARS.USDA.GOV>

Content-Type: text/plain;	charset="us-ascii"

HISTOTECHNOLOGIST RESEARCH FELLOWSHIP OPPORTUNITY - HISTOPATHOLOGY ON
FOREIGN ANIMAL DISEASES

A unique Histotechnologist research fellowship is currently available
working within the pathology division of the USDA, Animal Research
Service on Plum Island, NY. We have an immediate opening for a 1-2 year
fellow with the possibility of extension beyond the initial fellowship
contract.

The primary mission of the research group is investigation of veterinary
diseases of potential threat to US agriculture interests. Currently the
greatest emphasis is directed towards characterizing the pathogenesis of
foot-and-mouth disease (aphthovirus).

The Plum Island Animal Disease Center is located off the East end of
Long Island, NY. Transport to and from the island is provided free to
employees aboard government-operated ferries servicing Orient, NY and
Old Saybrook, CT. Rural and suburban communities are abundant near both
the NY and CT sides of the ferries with access to scenic beaches and
vineyards. New York City and Boston are both within 3-4hrs drive. 

Activities will be varied but will include conventional histotech work,
cryosectioning, immunohistochemistry, in-situ hybridization, confocal
microscopy, and assistance with animal necropsies and sample collection.
The pathology group currently consists of two veterinary pathologists
and one other histotech; the group works closely with on-site molecular
biologists, immunologists, clinicians, and research fellows. 

Stipends (ranging from $35,000 to $45,000 a year) will be determined
according to education and experience in the field. This fellowship is
open to applicants with at least a bachelor's degree and experience in
histotechnology.

General aspects of the fellowship are described and application is
available at:

www.orau.gov/piadc

For more information, email to the address below. If applying, please
notify J. Arzt directly in addition to filing the application with
ORISE/ORAU.

********************************************************
Jonathan Arzt DVM, MPVM, DACVP
Foreign Animal Disease Research Unit
USDA/ARS Plum Island Animal Disease Center
P.O. Box 848, Greenport NY 11944
631-323-3171(ph)
631-323-3006(fax)
Jonathan.Arzt <@t> ARS.USDA.GOV
********************************************************

------------------------------

Message: 5
Date: Tue, 28 Nov 2006 11:21:33 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] Job opening at PhenoPath Laboratories
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C191CC3D.DE0B%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"

Hi all. I would like to announce a job opening at PhenoPath Laboratories in
Seattle, WA. 

The opening is in the clinical IHC division for a part-time technologist
(0.75 FTE), day shift. Responsibilities may include performing IHC, IF,
molecular testing on patient samples and tissue sectioning (paraffin and
frozen). Participation in the development of new tests or technologies is
included. Quality control, safety, and preventive maintenance procedures and
documentation are required elements of this position as well.
Strong preference given to ASCP certified (or eligible) laboratory techs.
PhenoPath Laboratories is a national specialty pathology laboratory
committed to providing outstanding clinical and research services. We offer
an excellent compensation and benefits package, including 401K. Salary will
be determined based on the overall skills and background of the chosen
applicant. 

To apply, please e-mail or fax resume & cover letter to:

PhenoPath Laboratories
551 North 34th Street, Suite 100
Seattle, WA 98103
Fax: 206.374.9009
Email: ihctech <@t> phenopath.com
Web: www.phenopath.com
Employment applications available at www.phenopath.com

-------------------------------------------------------------------------
This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any 
unauthorized review, use, disclosure or distribution is prohibited. If 
you are not the intended recipient, please contact the sender by e-mail 
and destroy all copies of the original message, or you may call PhenoPath 
Laboratories, Seattle, WA U.S.A. at (206) 374-9000.

------------------------------

Message: 6
Date: Tue, 28 Nov 2006 11:27:46 -0800 (PST)
From: Melody Thiessen <mail2melody <@t> yahoo.com>
Subject: [Histonet] flourescent microscope
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20061128192746.23061.qmail <@t> web36604.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi there Histonetters!  It's been a while, but I have
a friend who is in the market for a flourescent
microscope; and needs to start pricing them for an
equipment grant.  She's looking for an upright (light
source from the top) possibly with a monitor/video
feed as well as the standard eyepieces.  She needs UV,
red, and blue filters as well.  Any leads are
appreciated.  Thanks!
Melody Thiessen

____________________________________________________________________________
________
Do you Yahoo!?
Everyone is raving about the all-new Yahoo! Mail beta.
http://new.mail.yahoo.com

------------------------------

Message: 7
Date: Tue, 28 Nov 2006 11:36:51 -0800
From: "Mark Elliott" <MElliott <@t> mrl.ubc.ca>
Subject: [Histonet] Cytochrome P450 antibody staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <456C1F53020000D60002C069 <@t> mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII

Hi 
Does anyone have any experience staining for cytochrome p450 in
paraffin embedded tissues?  We are looking at CYP1A1 specifically.  We
can't seem to get it to stain and are looking for any hints/ideas.  We
are using human lung tissue.
Thanks 
Mark

***CONFIDENTIALITY NOTICE***
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reply e-mail and delete the original and all copies from your system.

------------------------------

Message: 8
Date: Tue, 28 Nov 2006 20:47:34 +0000
From: "connie grubaugh" <conniegrubaugh <@t> hotmail.com>
Subject: RE: [Histonet] Richard Allan
To: omnivore98 <@t> yahoo.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY107-F284F7C01A3B2CEF1044F2DD6E50 <@t> phx.gbl>
Content-Type: text/plain; format=flowed

I have worked at a few different labs in California and Nevada and every 
experience with Richard Allen has always been good.

Connie Grubaugh
Las Vegas Nv.

>From: Heather Renko <omnivore98 <@t> yahoo.com>
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Richard Allan Date: Mon, 27 Nov 2006 16:41:14 -0800 
>(PST)
>
>Hello Fellow Histonetters:
>
>   I want to hear the good the bad and the ugly on your experience with 
>Richard Allan???
>
>   Concerned Customer
>
>
>---------------------------------
>Everyone is raving about the all-new Yahoo! Mail beta.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
MSN Shopping has everything on your holiday list. Get expert picks by style,

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------------------------------

Message: 9
Date: Tue, 28 Nov 2006 21:01:16 -0000
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re: macrophages/microglia in FFPE tissue
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000501c71330$589583a0$0201a8c0 <@t> carlba65530bda>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Hi Zach.
Perhaps you need to try heat-induced epitope retrieval/unmasking on you pwax

sections?
I have tried an OX-42 Ab reagent on FFPW sections after the above with 
negative results. ( and tried various proteolytic enzmes). The only 
excellent result , for me, was after HIER on pwax rat brain, using an 
anti-phosphotyrosine Ab reagent. See here for your evaluation; 
http://www.immunoportal.com/index.php Go to Image gallery and use search box

"phosphotyrosine". No guarantees for your species, of course.
Good luck.
carl 

------------------------------

Message: 10
Date: Tue, 28 Nov 2006 14:41:08 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] automated immuno stainer
To: <pathrm35 <@t> charter.net>, "'histonet'"
	<histonet <@t> pathology.swmed.edu>,	"'Patti Loykasek'"
	<ploykasek <@t> phenopath.com>,	"'Chistine Tambasco'"
	<immrstambo <@t> hotmail.com>
Message-ID: <004001c71335$eaf38210$6501a8c0 <@t> Patsy>
Content-Type: text/plain;	charset="us-ascii"

I agree with Patti.  
I have used Ventana, Biogenex and Dako IHC stainers in research and clinical
settings.  For cost effectiveness, consistent, reliable results I choose the
Dako.  I have had the same Dako rep for over 10 years and she is great
(LuAnne you know who I am talking about).  I did not have to pay for the
shipping of my instrument and use the instrument based on reagent
requisition.  We worked together for weeks validating the antibodies I use
and they even provided the reagents in some cases.  I know Dako has had some
troubles of late, but for my money and satisfaction they have and continue
to serve me well.
Patsy Ruegg 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
pathrm35 <@t> charter.net
Sent: Monday, November 27, 2006 8:49 PM
To: histonet; Patti Loykasek; Chistine Tambasco
Subject: Re: [Histonet] automated immuno stainer

I've used the Ventana, Biogenex and Vision Biosytem Bond IHC stainers. The
Vision and Ventana are more user friendly for online dewaxing and antigen
retrieval but I really did like the RTU antibodies from Biogenex.They were
very consistent and clean staining.  Vision Biosytem (Steve Westra in
Florida) did have the best technical support though.
At one place I worked, the Dako rep wanted to charge us $1,700.00 to ship a
demo IHC stainer for us to try. My MD didn't even consider Dako after that.
There is no perfect system. You just need to find the one that will fit your
needs and budget. I would suggest getting a solid price quote on the
equipment, antibodies and other disposable products prior to purchasing.

Ron Martin, BS, HT (ASCP) HTL, QIHC 
---- Patti Loykasek <ploykasek <@t> phenopath.com> wrote: 
> Hi Christine. I believe that a good, well trained tech can achieve good
> immunos on any type of platform. I think the main issues are your
> preferences: do you want an open or closed system - any detection system
or
> only the instrument company, concentrated or prediluted antibodies,etc?
> Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease
> of waste disposal? Can you use the clones of antibodies you have currently
> validated on the instrument? User friendly? Those are the main issues I
can
> think of at the moment - my brain is still tired from all the turkey
> tryptophan!  
> Most companies are more than happy to provide you with a demo & they may
> even help work up some things for you.
> As for my preference - it is Dako. We have several of the 48 slide
> configured Autostainers. I'd be happy to respond to any questions you
might
> have about the Dako.
> Good luck in your stainer hunting.
> 
> 
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
> 
> 
> 
> 
> 
> 
> 
> > Hello all! I hope everyone enjoyed their breif repreive from stress. I
was
> > wondering if anyone could give me some input on automated stainers for
> > immunohistochemistry. Currently we hand stain and our volumes are
steadily
> > increasing. We are looking at automation as we probably do up to 12-15
stains
> > per day and plan on starting ER and PR. I heard Vantana is very good.
How
> > about Leica?  (Theirs does both immunos and ordianry special stains.)
Anyone
> > have any experience they would like to share? We are an Ascension Health
> > affiliated hospital as that may matter in the negotiations. Thanks for
any and
> > all opinions.
> > Respectfullly,
> > Christine Tambasco, HT (ASCP)
> > St. Mary's Hospital
> > Amsterdam, New York 12010
> > 518-841-7287
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> -------------------------------------------------------------------------
> This e-mail message, including any attachments, is for the sole use of
> the intended recipients and may contain privileged information. Any 
> unauthorized review, use, disclosure or distribution is prohibited. If 
> you are not the intended recipient, please contact the sender by e-mail 
> and destroy all copies of the original message, or you may call PhenoPath 
> Laboratories, Seattle, WA U.S.A. at (206) 374-9000.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Tue, 28 Nov 2006 16:48:25 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] automated immuno stainer
To: "Patsy Ruegg" <pruegg <@t> ihctech.net>, <pathrm35 <@t> charter.net>,
	"histonet" <histonet <@t> pathology.swmed.edu>,	"Patti Loykasek"
	<ploykasek <@t> phenopath.com>,	"Chistine Tambasco"
	<immrstambo <@t> hotmail.com>
Message-ID:
	<1CD6831EB9B26D45B0A3EAA79F7EBD3203708566 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;	charset="us-ascii"

I second! - even with the problems of late, I have always had good
service from DAKO - all the way back to the early 80s, when I was very
green behind the ears and they walked me through the manual stains
holding my hands. They are a very good company. 

Joyce

Confidentiality Notice ** The information contained in this message may be
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addressee listed above. If you are neither the intended recipient nor the
employee or agent responsible for delivering this message to the intended
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information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer. Thank you. Saint Joseph's Health System,
Inc.

------------------------------

Message: 12
Date: Tue, 28 Nov 2006 18:23:53 -0600
From: "Rey, Jean-Philippe" <JPR <@t> Stowers-Institute.org>
Subject: [Histonet] Missouri Society for Histotechnologists rewards
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<CED81D34E37D5043A1211565277A51E5032FAE32 <@t> exchkc02.stowers-institute.org>

Content-Type: text/plain;	charset="iso-8859-1"

Hello everyone,

Does someone know one of these persons?
	-Bill DeSalvo
	-Chris Clark Casey
	-Jerry Fredenbergh
	-Elvie Taylor
	-Billie Carney
	-Ellen Ellis
I try to contact them concerning an award their received from the Missouri
Society for Histotechnologists between 1991 and 2006.
Thanks for you help!!

Jean-Philippe Rey 
Histology Specialist II
Histology Core Facility 
Stowers Institute for Medical Research 
1000 E. 50th St. 
Kansas City, MO  64110 
(816) 926-4305 

------------------------------

Message: 13
Date: Tue, 28 Nov 2006 16:42:57 -0800
From: "Comcast Mail" <tinayates <@t> comcast.net>
Subject: [Histonet] SEEKING PAUL HASSEMER
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <02af01c7134f$52f04290$71f9c147 <@t> DFC4J821>
Content-Type: text/plain;	charset="iso-8859-1"

Paul Hassemer, please contact me at your convenience.  Thanks.

Tina Yates, HT(ASCP)
Sr. Lab Specialist
Salem Hospital Regional Laboratory
P:  503.561.5443
F:  503.561.4781
tina.yates <@t> salemhospital.org

------------------------------

Message: 14
Date: Tue, 28 Nov 2006 20:11:36 -0500
From: "donna rossi" <djmr55 <@t> hotmail.com>
Subject: [Histonet] Room temperature
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY103-F1CC074BEB4D608AFF3909DEE40 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"

   Hi Everyone in Histoland!

   Can  someone please tell me what is the correct room temperature range
   for  a  histology lab that has an autoimmunostainer located within it.
   Also,   at  what  temperature  should  paraffin  blocks  be stored at?
   Thanks,  Donna ,SRHS
   [emwink.gif] donnarossi [emcocktl.gif]
     _________________________________________________________________

   [1]Stay  up-to-date  with your friends through the Windows Live Spaces
   friends list.

References

   1. http://g.msn.com/8HMBENUS/2737??PS=47575

------------------------------

Message: 15
Date: Tue, 28 Nov 2006 17:31:15 -0800 (PST)
From: Heather Renko <omnivore98 <@t> yahoo.com>
Subject: [Histonet] Thank you all for your feedback
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20061129013115.76267.qmail <@t> web31310.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

To all, thank you for your positive feedback regarding RA.  It really helped
me with my perspective on service for capital equipment.

  Heather

---------------------------------
Access over 1 million songs - Yahoo! Music Unlimited.

------------------------------

Message: 16
Date: Wed, 29 Nov 2006 15:39:51 +1100
From: stevenk <stevenk <@t> med.usyd.edu.au>
Subject: [Histonet] weils stain for myelin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <f06010200c192bd77d352@[129.78.38.216]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

	I am asking for a colleague who was doing a Weil's stain for myelin.

	One of the ingredients, 2% alcoholic haematoxylin requires a 
ripening process for at least 6 months.

	As the student cannot wait that long, I was wondering if 
anyone could supply a quicker method, or suggest something to hasten 
the ripening process of the above.

	Thanks from down under

	Stephen
-- 
|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Australia
|Ph: + 61 2 9351 6143
|Fax:+61 2 9351 3429

------------------------------

Message: 17
Date: Tue, 28 Nov 2006 21:54:35 -0800
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] weils stain for myelin
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01c7137a$d936bed0$130e4246 <@t> yourlk4rlmsu>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
	reply-type=response

Make up a fresh solution and just dust a little sodium iodate into it, but 
not too much.  Leave half an hour and use.

Alternatively, use a fresh solution and leave it ten minutes after adding 
the mordant.  It will ripen OK.  The method is done by inspection anyway, so

just keep an eye on the staining and adjust the times.

Bryan Llewellyn

----- Original Message ----- 
From: "stevenk" <stevenk <@t> med.usyd.edu.au>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 28, 2006 8:39 PM
Subject: [Histonet] weils stain for myelin

>
> I am asking for a colleague who was doing a Weil's stain for myelin.
>
> One of the ingredients, 2% alcoholic haematoxylin requires a ripening 
> process for at least 6 months.
>
> As the student cannot wait that long, I was wondering if anyone could 
> supply a quicker method, or suggest something to hasten the ripening 
> process of the above.
>
> Thanks from down under
>
> Stephen
> -- 
> |Stephen Kum Jew
> |Senior Technical Officer
> |Discipline of Pathology
> |School of Medical Sciences
> |Blackburn Building D06
> |University of Sydney NSW 2006
> |Australia
> |Ph: + 61 2 9351 6143
> |Fax:+61 2 9351 3429
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 18
Date: Wed, 29 Nov 2006 18:23:31 +1100
From: "Megan Clarke" <Megan.Clarke <@t> hnehealth.nsw.gov.au>
Subject: [Histonet] Orcein stain for fat in paraffin sections
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s56dd03d.064 <@t> domainatrix.HAHS.HEALTH.NSW.GOV.AU>
Content-Type: text/plain; charset=US-ASCII

Hi All

Hope that someone out there has this old stain procedure, an Orcein stain to
stain for fat in paraffin sections. We had tissue erronously placed in
formalin and subsequently processed. The pathologist would like to
demonstrate fat using the orcein method.

Thanks

Zenobia Haffajee
HAPS, IHC,
Anatomical pathology
Newcastle
Australia.

------------------------------

Message: 19
Date: Wed, 29 Nov 2006 12:40:06 +0200
From: Adi Sabag <adisabag <@t> techunix.technion.ac.il>
Subject: [Histonet] Anti GFP on cryosections
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1164796806.456d6386dedc5 <@t> webmail.technion.ac.il>
Content-Type: text/plain; charset=Windows-1255

Hi All, 

Does anyone have an experience with anti-GFP antibody of MBL on
cryosections?

Thanks- 

Adi

-- 
Adi Sabag
Tumor Progression and Angiogenesis laboratory
Medicine Faculty, Technion
Haifa, Israel. 
972-4-8295425

------------------------------

Message: 20
Date: Wed, 29 Nov 2006 12:20:59 +0100
From: "Brunella Spaggiari" <brunella.spaggiari <@t> nemo.unipr.it>
Subject: [Histonet] Von Kossa stain
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003301c713a8$72cd5040$ba3a4ea0 <@t> PC186Gabbi>
Content-Type: text/plain; charset="iso-8859-1"

We work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and
embedded in methylmethacrilate (MMA).
>From our samples we obtain 50 m thick sections which can contain titanium
implants.
We tried to perform Von Kossa stain (see attachment), always on
undeplasticized sections, but we met several problems:

- calcium deposits after silver nitrate+UV light did not appear black but
brown, and, after sodium thiosulphate, brown stain turns more and more
light;

- we could not find a suitable counterstain for osteoid: nuclear fast red
and neutral red do not stain at all, basic or acid fuchsin stain too much
and cover the underlying brown..

Some suggestions? Are there other good histological stainings for
undeplasticized bone sections in your experience?

Thank you!
Brunella

------------------------------

Message: 21
Date: Wed, 29 Nov 2006 06:19:36 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] FW: controls
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<0E394B648E5284478A6CCB78E5AFDA27036407AC <@t> hpes1.HealthPartners.int>
Content-Type: text/plain;	charset="us-ascii"

Trying this message again, as we still need to come up with a good
control for ALK-1!!

>  -----Original Message-----
> From: 	Webb, Dorothy L  
> Sent:	Tuesday, November 14, 2006 10:52 AM
> To:	'Histonet <@t> lists.utsouthwestern.edu'
> Subject:	controls
> 
> Does anyone know of a possible source to purchase ALK-1 controls?  We
> cannot seem to come up with a good tissue control and have used
> CellMarque as our source, but, they are not available through them at
> this time.  Appreciate any help in this area and thanks ahead of
> time!!
________________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please be
advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is strictly
prohibited.

If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will be
reimbursed for reasonable costs incurred in notifying us.

------------------------------

Message: 22
Date: Wed, 29 Nov 2006 05:31:20 -0800 (PST)
From: Coleman Manufacturing <coleman_manufacturing <@t> yahoo.com>
Subject: [Histonet] Fwd: Fwd: FW: READ!!!!!!!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20061129133120.96227.qmail <@t> web37606.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

  Date: Tue, 28 Nov 2006 23:17:46 -0500
From: "Sara L. Browder" <browdesl <@t> mailbox.sc.edu>
To: coleman_manufacturing <@t> yahoo.com
Subject: Fwd: Fwd: FW: READ!!!!!!!

----- Forwarded message from steelea3 <@t> mailbox.sc.edu -----
Date: Tue, 28 Nov 2006 20:31:49 -0500
From: steelea3 <@t> mailbox.sc.edu
Reply-To: steelea3 <@t> mailbox.sc.edu
Subject: Fwd: FW: READ!!!!!!!
To: browdesl <@t> mailbox.sc.edu

----- Forwarded message from kkyle lavette -----
Date: Wed, 29 Nov 2006 00:56:44 +0000
From: kkyle lavette 
Reply-To: kkyle lavette 
Subject: FW: READ!!!!!!!
To: bucablack2 <@t> aol.com, MusicalSMJ <@t> aol.com, robtbarton <@t> aol.com, 
DonnaB210 <@t> aol.com, braziel <@t> webmail.sc.edu, xxraiderxx72 <@t> hotmail.com, 
conleydarrick <@t> yahoo.com, daiglep <@t> mailbox.sc.edu, DAVISKE <@t> gwm.sc.edu, 
johnson <@t> cadetmail.uscga.edu, canesnj8 <@t> aol.com, ashkirk09 <@t> yahoo.com, 
G_Knight_21 <@t> hotmail.com, jimv <@t> cox.net, jpvasellina <@t> hotmail.com, 
jvas <@t> bellsouth.net, STEELEA3 <@t> mailbox.sc.edu, napole5127 <@t> aol.com, 
rose.shores <@t> pw.utc.com, kels1021 <@t> hotmail.com, mike787b <@t> hotmail.com, 
johnnyv <@t> ntplx.net, Xaviar3 <@t> aol.com, mckier <@t> mailbox.sc.edu, 
idodough <@t> hotmail.com, dlavette <@t> ofalaw.com

>From: HANDKL <@t> mailbox.sc.edu
>To: HANDKL <@t> mailbox.sc.edu
>Subject: READ!!!!!!!
>Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST)
>
>Read carefully...
>
>
>THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL
>
>To all of my friends, I do not usually forward messages,
>But this is from my friend Pearlas Sandborn and she really is
>an attorney.
>
>If she says that this will work - It will work. After all,What have
>you got to lose?
>
>SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an
>attorney, And I know the law. This thing is for real. Rest assured
>AOL and &nbs p; Intel will follow through with their promises for
>fear of facing a multimillion-dollar class action suit similar to the one
>filed by PepsiCo against General Electric not too long ago.
>
>Dear Friends: Please do not take this for a junk letter.
>Bill Gates sharing his fortune. If you ignore this, You will repent
>later.
>
>Microsoft and AOL are now the largest Internet companies
>and in an effort to make sure that Internet Explorer remains the
>most widely used program, Microsoft and AOL are running an e-mail
>beta test.
>
>When you forward this e-mail to friends, Microsoft can and will
>track it (If you are a Microsoft Windows user) For a two weeks
>time period.
>
>For every person that you forward this e-mail to, Microsoft will pay
>you $245.00 For every person that you sent it to that forwards it on,
>Microsoft will pay you $243.00 and for every third person that receives
>it, You will be paid $241.00. Within two weeks, Microsoft will contact
>you for your address and then send you a check.
>
>Regards. Charles S Bailey General Manager Field Operations
>1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085
>
>
>Thought this was a scam myself, But two weeks after receiving this
>e-mail and forwarding it on. Microsoft contacted me for my address and
>within days, I received a check for $24, 800.00. You need to respond
>before the beta testing is over. If anyone can affoard this, Bill gates is 
>the
>man.
>
>It's all marketing expense to him. Please forward this to as many
>people as possible. You are bound to get at least $10, 000.00
>We're not going to help them out with their e-mail beta test without
>getting a little something for our time. My brother's girlfriend got in
>on this a few months ago. When I went to visit him for the Baylor/UT
>game, she showed me her check. It was for the sum of $4, 324.44 and
>was stamped "Paid In Full".
>
>
>
>
>
>
>
>
>
>
>_____________________ Confidentiality _____________________
>
>
>
>This electronic transmission is strictly confidential and intended
>
>solely for the addressee. It may contain information which is covered
>
>by legal, professional or other privilege. If you are not the intended
>
>addressee, you must not disclose, copy or take any action in reliance
>
>of this transmission. If you have received this transmission in error,
>
>please notify us and delete the received data as soon as possible.
>
>
>
>This footnote also confirms that this email message has been swept
>
>for the presence of computer viruses.
>
>_______________________________________________________
>
>
>
>
>
>
>
> Adalgiza "ADA" Storto
>
>A & D SupportWear Inc.
>
>5919 Greenville Ave. #380
>
> Dallas,TX 75206
>
> 214-883-7373
>
> office: 214-363-1600 / 972- 414- 3701
>
> fax: 214-265-7655 / 972- 414-3718
>
>e-mail: adarx2000 <@t> yahoo.com
>
>
>
>
>
>
>
>
>
>---------------------------------------------------------------------------
---
-
-
>
>
>
>
>
>IMPORTANT: The sender intends that this electronic message is for
>the exclusive use of the person to whom it is addressed. This
>message may contain information that is confidential. If the reader
>of this message is not an intended recipient, be aware that any
>disclosure, dissemination, distribution or copying of this
>communication, or the use of its contents, is prohibited. If you
>have received this message in error, then please immediately notify
>the sender of your inadvertent receipt and delete this message from
>all data storage systems. Thank you.

_________________________________________________________________
Fixing up the home? Live Search can help 
http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=e
n-
US&source=hmemailtaglinenov06&FORM=WLMTAG

----- End forwarded message -----

----- End forwarded message -----

Sara L. Browder

------------------------------

Message: 23
Date: Wed, 29 Nov 2006 07:58:33 -0600
From: "Lester Raff" <LRaff <@t> lab.uropartners.com>
Subject: [Histonet] Don't fall for the Hoax in a just posted histonet
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5DA1CA5D0B98A84985B545A24423B822019ADA <@t> UPLAB01.uplab.local>
Content-Type: text/plain;	charset="us-ascii"

See below before you expect any of Bill Gates' money.

http://www.hoax-slayer.com/ms-money-giveway-hoax.html

Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, IL 60154

ph:  708-486-0076
fax: 708-486-0080

------------------------------

Message: 24
Date: Wed, 29 Nov 2006 08:17:36 -0600
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] Don't fall for the Hoax in a just posted
	histonet
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<B3D65550856D0146B900D401EE313D4B641369 <@t> dynams.dynacaremilwaukee.com>
Content-Type: text/plain;	charset="iso-8859-1"

It never ceases to amaze that people still fall for this garbage.  WOW.

I just wish they would come up with something more original like an upcoming
diamond meteor shower or a new laser that turns used kleenex into gold.

Gulliblely Yours,

Glen Dawson
Milwaukee, WI

------------------------------

Message: 25
Date: Wed, 29 Nov 2006 09:34:43 -0500
From: Anne C Lewin <anne.lewin <@t> bms.com>
Subject: Re: [Histonet] Fwd: Fwd: FW: READ!!!!!!!
To: Coleman Manufacturing <coleman_manufacturing <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <456D9A83.1040207 <@t> bms.com>
Content-Type: text/plain; charset="iso-8859-1"

*This is a hoax*, started in 1997
for more info, visit Snopes.com, they are great when it comes to 
de-mything urban legends......

http://www.snopes.com/inboxer/nothing/microsoft-aol.asp

Coleman Manufacturing wrote:

>  Date: Tue, 28 Nov 2006 23:17:46 -0500
>From: "Sara L. Browder" <browdesl <@t> mailbox.sc.edu>
>To: coleman_manufacturing <@t> yahoo.com
>Subject: Fwd: Fwd: FW: READ!!!!!!!
>
>
>
>----- Forwarded message from steelea3 <@t> mailbox.sc.edu -----
>Date: Tue, 28 Nov 2006 20:31:49 -0500
>From: steelea3 <@t> mailbox.sc.edu
>Reply-To: steelea3 <@t> mailbox.sc.edu
>Subject: Fwd: FW: READ!!!!!!!
>To: browdesl <@t> mailbox.sc.edu
>
>
>
>----- Forwarded message from kkyle lavette -----
>Date: Wed, 29 Nov 2006 00:56:44 +0000
>From: kkyle lavette 
>Reply-To: kkyle lavette 
>Subject: FW: READ!!!!!!!
>To: bucablack2 <@t> aol.com, MusicalSMJ <@t> aol.com, robtbarton <@t> aol.com, 
>DonnaB210 <@t> aol.com, braziel <@t> webmail.sc.edu, xxraiderxx72 <@t> hotmail.com, 
>conleydarrick <@t> yahoo.com, daiglep <@t> mailbox.sc.edu, DAVISKE <@t> gwm.sc.edu, 
>johnson <@t> cadetmail.uscga.edu, canesnj8 <@t> aol.com, ashkirk09 <@t> yahoo.com, 
>G_Knight_21 <@t> hotmail.com, jimv <@t> cox.net, jpvasellina <@t> hotmail.com, 
>jvas <@t> bellsouth.net, STEELEA3 <@t> mailbox.sc.edu, napole5127 <@t> aol.com, 
>rose.shores <@t> pw.utc.com, kels1021 <@t> hotmail.com, mike787b <@t> hotmail.com, 
>johnnyv <@t> ntplx.net, Xaviar3 <@t> aol.com, mckier <@t> mailbox.sc.edu, 
>idodough <@t> hotmail.com, dlavette <@t> ofalaw.com
>
>
>
>
>  
>
>>From: HANDKL <@t> mailbox.sc.edu
>>To: HANDKL <@t> mailbox.sc.edu
>>Subject: READ!!!!!!!
>>Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST)
>>
>>Read carefully...
>>
>>
>>THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL
>>
>>To all of my friends, I do not usually forward messages,
>>But this is from my friend Pearlas Sandborn and she really is
>>an attorney.
>>
>>If she says that this will work - It will work. After all,What have
>>you got to lose?
>>
>>SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an
>>attorney, And I know the law. This thing is for real. Rest assured
>>AOL and &nbs p; Intel will follow through with their promises for
>>fear of facing a multimillion-dollar class action suit similar to the one
>>filed by PepsiCo against General Electric not too long ago.
>>
>>Dear Friends: Please do not take this for a junk letter.
>>Bill Gates sharing his fortune. If you ignore this, You will repent
>>later.
>>
>>Microsoft and AOL are now the largest Internet companies
>>and in an effort to make sure that Internet Explorer remains the
>>most widely used program, Microsoft and AOL are running an e-mail
>>beta test.
>>
>>When you forward this e-mail to friends, Microsoft can and will
>>track it (If you are a Microsoft Windows user) For a two weeks
>>time period.
>>
>>For every person that you forward this e-mail to, Microsoft will pay
>>you $245.00 For every person that you sent it to that forwards it on,
>>Microsoft will pay you $243.00 and for every third person that receives
>>it, You will be paid $241.00. Within two weeks, Microsoft will contact
>>you for your address and then send you a check.
>>
>>Regards. Charles S Bailey General Manager Field Operations
>>1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085
>>
>>
>>Thought this was a scam myself, But two weeks after receiving this
>>e-mail and forwarding it on. Microsoft contacted me for my address and
>>within days, I received a check for $24, 800.00. You need to respond
>>before the beta testing is over. If anyone can affoard this, Bill gates is

>>the
>>man.
>>
>>It's all marketing expense to him. Please forward this to as many
>>people as possible. You are bound to get at least $10, 000.00
>>We're not going to help them out with their e-mail beta test without
>>getting a little something for our time. My brother's girlfriend got in
>>on this a few months ago. When I went to visit him for the Baylor/UT
>>game, she showed me her check. It was for the sum of $4, 324.44 and
>>was stamped "Paid In Full".
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>_____________________ Confidentiality _____________________
>>
>>
>>
>>This electronic transmission is strictly confidential and intended
>>
>>solely for the addressee. It may contain information which is covered
>>
>>by legal, professional or other privilege. If you are not the intended
>>
>>addressee, you must not disclose, copy or take any action in reliance
>>
>>of this transmission. If you have received this transmission in error,
>>
>>please notify us and delete the received data as soon as possible.
>>
>>
>>
>>This footnote also confirms that this email message has been swept
>>
>>for the presence of computer viruses.
>>
>>_______________________________________________________
>>
>>
>>
>>
>>
>>
>>
>>Adalgiza "ADA" Storto
>>
>>A & D SupportWear Inc.
>>
>>5919 Greenville Ave. #380
>>
>>Dallas,TX 75206
>>
>>214-883-7373
>>
>>office: 214-363-1600 / 972- 414- 3701
>>
>>fax: 214-265-7655 / 972- 414-3718
>>
>>e-mail: adarx2000 <@t> yahoo.com
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>--------------------------------------------------------------------------
----
>>    
>>
>-
>-
>  
>
>>
>>
>>
>>IMPORTANT: The sender intends that this electronic message is for
>>the exclusive use of the person to whom it is addressed. This
>>message may contain information that is confidential. If the reader
>>of this message is not an intended recipient, be aware that any
>>disclosure, dissemination, distribution or copying of this
>>communication, or the use of its contents, is prohibited. If you
>>have received this message in error, then please immediately notify
>>the sender of your inadvertent receipt and delete this message from
>>all data storage systems. Thank you.
>>    
>>
>
>_________________________________________________________________
>Fixing up the home? Live Search can help 
>http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=
en-
>US&source=hmemailtaglinenov06&FORM=WLMTAG
>
>
>----- End forwarded message -----
>
>
>
>----- End forwarded message -----
>
>
>Sara L. Browder
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 36, Issue 34
****************************************