[Histonet] weils stain for myelin
Nicholas_Gidmark <@t> brown.edu
Wed Nov 29 09:07:07 CST 2006
I've used Erlich's haematoxylin and my recipe has 0.5 grams of sodium iodate as a good instant-ripener for 600ml of solution, just to give you an idea of scale.
Nicholas J. Gidmark, graduate student
Brainerd lab of biomechanics and functional morphology
Dept. of Ecology & Evolutionary Biology
80 Waterman Street, box G-W
Providence, RI 02912
nicholas_gidmark <@t> brown.edu
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of stevenk
Sent: Tue 28-Nov-06 11:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] weils stain for myelin
I am asking for a colleague who was doing a Weil's stain for myelin.
One of the ingredients, 2% alcoholic haematoxylin requires a
ripening process for at least 6 months.
As the student cannot wait that long, I was wondering if
anyone could supply a quicker method, or suggest something to hasten
the ripening process of the above.
Thanks from down under
|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Ph: + 61 2 9351 6143
|Fax:+61 2 9351 3429
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