[Histonet] weils stain for myelin

[Bryan Llewellyn]

Make up a fresh solution and just dust a little sodium iodate into it, but 
not too much.  Leave half an hour and use.

Alternatively, use a fresh solution and leave it ten minutes after adding 
the mordant.  It will ripen OK.  The method is done by inspection anyway, so 
just keep an eye on the staining and adjust the times.

Bryan Llewellyn


----- Original Message ----- 
From: "stevenk" <stevenk <@t> med.usyd.edu.au>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 28, 2006 8:39 PM
Subject: [Histonet] weils stain for myelin


>
> I am asking for a colleague who was doing a Weil's stain for myelin.
>
> One of the ingredients, 2% alcoholic haematoxylin requires a ripening 
> process for at least 6 months.
>
> As the student cannot wait that long, I was wondering if anyone could 
> supply a quicker method, or suggest something to hasten the ripening 
> process of the above.
>
> Thanks from down under
>
> Stephen
> -- 
> |Stephen Kum Jew
> |Senior Technical Officer
> |Discipline of Pathology
> |School of Medical Sciences
> |Blackburn Building D06
> |University of Sydney NSW 2006
> |Australia
> |Ph: + 61 2 9351 6143
> |Fax:+61 2 9351 3429
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>