[Histonet] Hexane freezing Method for Cryosectioning

[Markus F. Meyenhofer]

Why solvents? To counter the "Leidenfrost Effect" . Google or wikipedia for 
the explanation>
Markus
----- Original Message ----- 
From: "patsy ruegg" <pruegg <@t> ihctech.net>
To: <pmarcum <@t> vet.upenn.edu>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, November 24, 2006 4:25 PM
Subject: RE: [Histonet] Hexane freezing Method for Cryosectioning


> Pam,
> Hexane is still very flammable, not sure if it is any less explosive, both
> would probably be considered pretty hazardous.  If you are using liquid
> nitrogen why do you also need the solvent?  I snap freeze by putting oct
> around the sample in a cryo mold, then slowly lower into liquid nitrogen
> with good results for frozen sections on undecalcified bone, better yet 
> for
> tape transfer sections, I fix, wash and infiltrate over night at 4dc in 
> 30%
> sucrose, then do the snap freezing with OCT and liquid nitrogen.  John
> Trapley wrote an article for JOH on this several years ago.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> pmarcum <@t> vet.upenn.edu
> Sent: Friday, November 24, 2006 11:49 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hexane freezing Method for Cryosectioning
>
> Good Afternoon,
>
> I have been asked to look into using hexane in rapid freezing of tissues
> (primarily non-decalcified bone).  I am looking it up with several other
> searches and wondered if anyone is currently using this technique.  Is it
> better or safer than isopentane with liquid nitrogen?  I know the issues
> with
> isopentane and explosions so this partly a safety questions also.
>
> Thanks for the help in advance.
>
> Pam Marcum
> UPENN Vet School
> New Bolton Center
>
>
>
>
>
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