[Histonet] Hexane freezing Method for Cryosectioning

[patsy ruegg]

Pam,
Hexane is still very flammable, not sure if it is any less explosive, both
would probably be considered pretty hazardous.  If you are using liquid
nitrogen why do you also need the solvent?  I snap freeze by putting oct
around the sample in a cryo mold, then slowly lower into liquid nitrogen
with good results for frozen sections on undecalcified bone, better yet for
tape transfer sections, I fix, wash and infiltrate over night at 4dc in 30%
sucrose, then do the snap freezing with OCT and liquid nitrogen.  John
Trapley wrote an article for JOH on this several years ago.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
pmarcum <@t> vet.upenn.edu
Sent: Friday, November 24, 2006 11:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Hexane freezing Method for Cryosectioning

Good Afternoon,

I have been asked to look into using hexane in rapid freezing of tissues
(primarily non-decalcified bone).  I am looking it up with several other
searches and wondered if anyone is currently using this technique.  Is it
better or safer than isopentane with liquid nitrogen?  I know the issues
with
isopentane and explosions so this partly a safety questions also.

Thanks for the help in advance.

Pam Marcum
UPENN Vet School
New Bolton Center





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