[Histonet] Toluene and Flouresence Staining
jkiernan <@t> uwo.ca
Sat Nov 4 22:52:51 CST 2006
Send a detailed account of your technique.
You will many very helpful replies.
sohail ejaz wrote:
> I am studying tracheal vasculature by using vWF as blood vessel marker.
> After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................??
> Can anyone guide me for to slove this issue.
> If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that.
> Dr. Rudiger Tom
> University of Connecticut, Storrs
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