[Histonet] Toluene and Flouresence Staining

[sohail ejaz]

I am studying tracheal vasculature by using vWF as blood vessel marker.
  After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................??
   
  Can anyone guide me for to slove this issue.
   
  If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that.
   
  Regard
   
  Dr. Rudiger Tom
  University of Connecticut, Storrs
  Connecticut

 
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