[Histonet] whole villi

[Geoff McAuliffe]

The tissue curls due to the smooth muscle in the wall of the gut. Pin 
the tissue out flat on a piece of cork sheet (available at Home Depot or 
Lowe's) then float, tissue side down, in fixative.

Geoff

Till, Renee wrote:

>I sometimes work with rat and mouse gi tissues, and as you might expect
>one of the most time consuming things is getting the tissues embedded
>good. The colon has not been as much of a problem, but lately we are
>doing more with the jejunum and getting whole villi is enough to make me
>scream. It seems to me that one of my main problems is that sometime
>between the collection of the tissues (which is not done by me) and when
>I embed them, the tissues often curl up and it is hard to get a good
>straight piece to embed. We embed them on end? (so when cut it makes a
>little O). Any ideas on how to keep them straight during the processing?
>Or maybe if there is something else that I am not thinking of that could
>be a problem. Thanks.
>
> 
>
>Renee' Till, HT
>
>Research Assistant
>
>Arkansas Children's Nutrition Center
>
>1212 Marshall St./N2021
>
>Little Rock, AR 72002
>
>Office (501)364-2785
>
>Fax (501)364-3161
>
> 
>
>
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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