[Histonet] whole villi

[Till, Renee]

I sometimes work with rat and mouse gi tissues, and as you might expect
one of the most time consuming things is getting the tissues embedded
good. The colon has not been as much of a problem, but lately we are
doing more with the jejunum and getting whole villi is enough to make me
scream. It seems to me that one of my main problems is that sometime
between the collection of the tissues (which is not done by me) and when
I embed them, the tissues often curl up and it is hard to get a good
straight piece to embed. We embed them on end? (so when cut it makes a
little O). Any ideas on how to keep them straight during the processing?
Or maybe if there is something else that I am not thinking of that could
be a problem. Thanks.

 

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72002

Office (501)364-2785

Fax (501)364-3161

 


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