[Histonet] DNA/RNA Extraction Kits for paraffinised tissues

[Michael J. Lyon, Ph.D.]

I just receive information from Stratagene.  They have a kit "Absolutely RNA
FFPE Kit".  www.statagene.com

I have not used it so have no knowledge to its usefulness.

Mike

> -----Original Message-----
> From: louise renton [mailto:louise.renton <@t> gmail.com]
> Sent: Tuesday, May 23, 2006 4:36 AM
> To: Shirley PHUA
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues
> 
> Dear Shirley,
> 
> My information may be somewhat out of date as it is several years
> since I did DNA extration on formalin fixed /praffin embedded tissues,
> but at that time I found that the commercial kits (specifically the
> ones using spin columns) were not as good as the old fashioned method
> of phenol chloroform.
> 
> The amount/quality of DNA available is directly related to the type
> and length of fixation so harsh unbuffered fixation will degrade the
> DNA.
> 
> This is my 2 cents worth
> 
> 
> On 5/23/06, Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg> wrote:
> > Dear All
> >
> > Have anyone heard of any DNA/RNA extraction kits for formalin-fixed,
> > paraffinised tissues? I will greatly appreciate if you can share some of
> > your feedbacks. Were your yields good?
> >
> > Thanks in advance!
> >
> > Shirley Phua
> > Histopathology Laboratory
> > Centre for Forensic Medicine
> > Health Sciences Authority
> > DID : 62130654
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> >
> 
> 
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
>