[Histonet] DNA/RNA Extraction Kits for paraffinised tissues

[louise renton]

Dear Shirley,

My information may be somewhat out of date as it is several years
since I did DNA extration on formalin fixed /praffin embedded tissues,
but at that time I found that the commercial kits (specifically the
ones using spin columns) were not as good as the old fashioned method
of phenol chloroform.

The amount/quality of DNA available is directly related to the type
and length of fixation so harsh unbuffered fixation will degrade the
DNA.

This is my 2 cents worth


On 5/23/06, Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg> wrote:
> Dear All
>
> Have anyone heard of any DNA/RNA extraction kits for formalin-fixed,
> paraffinised tissues? I will greatly appreciate if you can share some of
> your feedbacks. Were your yields good?
>
> Thanks in advance!
>
> Shirley Phua
> Histopathology Laboratory
> Centre for Forensic Medicine
> Health Sciences Authority
> DID : 62130654
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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin