[Histonet] IHC on Cheek cells

[Tony Henwood]

Yep,

Though I would use a cell culture fluid like Hanks rather than PBS.
Seems to preserve the cells better.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Thursday, 11 May 2006 2:46 AM
To: yan gao; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC on Cheek cells


We used wooden tongue depressors to scrape cheeks for buccal smears -
that 
way you do not have cells trapped in cotton fibers.  There are scrapers,

plastic that may work better, you can still resuspend the cells off the 
ends - agitate the tubes containing scraper with a vortex mixer to let 
cells go into PBS.

I think you could cytospin the cells onto slides easily.

  At 09:45 AM 5/10/2006, you wrote:

>   Hi, Histonet.
>
>   I  am  staining  phosoph-MEK  on  cheek  cells.  Here is the way how
I
>   collected  cheek  cells.   I  used  cotton tips scrape inside of
human
>   mouth.   then  I  soak  the  tip  into  the fixative offered by
Thermo
>   shandon for cytospin.  Then I cytospined cells to slides.
>   While  I  stained  pMEK,  I  brought a rabbit IgG with it for
negative
>   control.   I  got  a  positive  stain on cheek cells.  Cells were
very
>   sticky.
>   Anyone  has IHC experience on buccal cells would like share with me,
I
>   really appreciated.  Thanks a lot.
>
>   Yan Gao
>
>   Novartis
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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