[Histonet] IHC on Cheek cells

[Tony Henwood]

Rabbit antibodies are notorious for having a natural antibody population
directed against human keratins. I would recommend absorbing the tissues
with human keratin extracts. There was a paper some time ago (?10 years)
that described this. Unfortunately I can't find it (These days I have
problems finding my way home!!)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of yan gao
Sent: Thursday, 11 May 2006 1:46 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on Cheek cells



   Hi, Histonet.

   I  am  staining  phosoph-MEK  on  cheek  cells.  Here is the way how
I
   collected  cheek  cells.   I  used  cotton tips scrape inside of
human
   mouth.   then  I  soak  the  tip  into  the fixative offered by
Thermo
   shandon for cytospin.  Then I cytospined cells to slides.
   While  I  stained  pMEK,  I  brought a rabbit IgG with it for
negative
   control.   I  got  a  positive  stain on cheek cells.  Cells were
very
   sticky.
   Anyone  has IHC experience on buccal cells would like share with me,
I
   really appreciated.  Thanks a lot.

   Yan Gao

   Novartis
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