[Histonet] insect histology book inquiry

Rittman, Barry R Barry.R.Rittman <@t> uth.tmc.edu
Thu Mar 30 09:27:16 CST 2006


Andi
I think that the N-butyl alcohol method is OK and some original methods
used this because it is miscible with paraffin wax. However if an
intermediary agent is not used then although the tissue remains softer
than when using ethanol,  the butyl alcohol takes much longer to be
replaced by the paraffin wax. 
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Thursday, March 30, 2006 9:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] insect histology book inquiry

Good Thursday morning!
Is there anybody out there in histoland who might have a copy of the now

out of print book, A Manual of Basic Techniques in Insect Histology by
P. 
Barbosa, Autumn Publishers, 1974?
I'm looking for details on Stile's butyl alcohol technique that was 
discussed in a paper on whiteflies and Barbosa was cited.
A jillion thanks to anybody who can provide this technique!
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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