[Histonet] tryptase IHC
Patti Loykasek
ploykasek <@t> phenopath.com
Fri Mar 24 10:07:27 CST 2006
Maria,
We are using a different clone - clone G3 from Chemicon. In our validation
we did see some cross reactivity with basophils. It was positive on all
cases of mast cell disease we tested & negative on other neoplasms &
hematopoietic cell types.
Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA
> Hello!
>
> We are having some trouble with our IHC stain for mast cell tryptase
> (Dako AA1 clone). We seem to get granular intracytoplasmic staining
> in several cells that are probably not mast cells but histiocytes or
> neoplastic plasma cells. I know that the antibody is very specific
> for mast cells and should not label anything else. So, does anybody
> have any ideas? Could it be the retrieval? Or the secondary? We use
> EnVision so it should not be a problem.
>
> Does anybody have any suggestions or has encounter a similar problem?
>
> Maria Forzan
>
> Finn Pathologists
>
> 44 (0)1379 854180
>
> "Pebbles are inevitable"
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