[Histonet] tryptase IHC

[maria forzan]

   Hello!

   We  are  having some trouble with our IHC stain for mast cell tryptase
   (Dako  AA1  clone).  We seem to get granular intracytoplasmic staining
   in  several  cells that are probably not mast cells but histiocytes or
   neoplastic  plasma  cells.   I know that the antibody is very specific
   for  mast  cells and should not label anything else.  So, does anybody
   have any ideas?  Could it be the retrieval?  Or the secondary?  We use
   EnVision so it should not be a problem.

   Does anybody have any suggestions or has encounter a similar problem?

   Maria Forzan

   Finn Pathologists

   44 (0)1379 854180

   "Pebbles are inevitable"