[Histonet] RE: mucicarmine

[Osborn, Sharon]

Dorothy,
	I did the mucicarmine for a year on cryptococcus research
project.  Therefore, I have a procedure that works great.  Basically, it
is the American Master Tech Scientific Muci-10 Procedure with
modifications that we found made improvements to the staining procedure.
If interested, I am happy to send the procedure to you.  We kept the
Muci-10 concentrate in the refrigerator and let it come to room Temp
before use.

Sharon Osborn
DNAX, SP BioPharma
Palo Alto, CA
650.496.6539


Message: 5
Date: Mon, 20 Mar 2006 09:28:54 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] mucicarmine
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<0E394B648E5284478A6CCB78E5AFDA270171FCE1 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain;	charset="US-ASCII"

We have been doing our mucicarmine on the Nexus special stainer and due
to the fact that we do not do a lot of these, I decided to bring it back
to handstaining.  I am using the Southgate's modification of Mayer's
Technique and having a very hard time getting the stain to look nice and
sharp!  Does anyone have any procedures they use or suggestions as to
what could be our problem?  Our muci is not staining the bright red and
even the nuclei are not stained very dark.  We have tried various times
and strengths of the mucicarmine and even from two different companies!
Help and thanks for any and all suggestions! _


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