[Histonet] H2DCFDA staining nuclear or perinuclear?

[Glenn Krasinski]

I have already bugged the flowers today with my H2DCFDA saga. Now this question is for my brothers/sisters on Histonet.  
   
  We are noting staining with H2DCFDA to be nuclear and perinuclear in A431 cells both untreated and treated with compound. (and not super pretty I might add). In Li et al JBC Vol. 281, No.3, pp.1495-1505.  There are some ultra pretty pictures showing it to be perinucluear at a similar concnetration (10uM H2DCFDA).  Any ideas? Comments? Personal experiences?
   
  Many thanks.
   
  Glenn M. Krasinski
  Flow Jock in Retirement
  San Diego, CA

		
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