[Histonet] T Cell Heat retrieval using microwave pressure cooker

[Joanne Mauger]

Diana,

Try using EDTA buffer (pH 8) for retrieval. It works for us!
Jo

>>> "Diana McCaig" <dmccaig <@t> ckha.on.ca> 03/15/06 11:33 AM >>>

Hi
I am trying to demonstrate T cells using Polyclonal Rabbit Anti human
CD3, T cell (DAKO ENVISION).  The control tissue is tonsil and using a
manual procedure.  I have increased the time in the microwave pressure
cooker from 5 up to 30 minutes (pH 6 buffer) and have no success.  The
antibody is incubated for 30 minutes (recommended) but I have gone as
high as 1 hour.  I am using AEC and Mayers counterstain.  Any help or
advise would be greatly appreciated.
				Diana 

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