[Histonet] T Cell Heat retrieval using microwave pressure cooker
mauger <@t> email.chop.edu
Wed Mar 15 12:04:59 CST 2006
Try using EDTA buffer (pH 8) for retrieval. It works for us!
>>> "Diana McCaig" <dmccaig <@t> ckha.on.ca> 03/15/06 11:33 AM >>>
I am trying to demonstrate T cells using Polyclonal Rabbit Anti human
CD3, T cell (DAKO ENVISION). The control tissue is tonsil and using a
manual procedure. I have increased the time in the microwave pressure
cooker from 5 up to 30 minutes (pH 6 buffer) and have no success. The
antibody is incubated for 30 minutes (recommended) but I have gone as
high as 1 hour. I am using AEC and Mayers counterstain. Any help or
advise would be greatly appreciated.
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