[Histonet] T Cell Heat retrieval using microwave pressure cooker

Joanne Mauger mauger <@t> email.chop.edu
Wed Mar 15 12:04:59 CST 2006


Try using EDTA buffer (pH 8) for retrieval. It works for us!

>>> "Diana McCaig" <dmccaig <@t> ckha.on.ca> 03/15/06 11:33 AM >>>

I am trying to demonstrate T cells using Polyclonal Rabbit Anti human
CD3, T cell (DAKO ENVISION).  The control tissue is tonsil and using a
manual procedure.  I have increased the time in the microwave pressure
cooker from 5 up to 30 minutes (pH 6 buffer) and have no success.  The
antibody is incubated for 30 minutes (recommended) but I have gone as
high as 1 hour.  I am using AEC and Mayers counterstain.  Any help or
advise would be greatly appreciated.

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