[Histonet] Mayer's Hematoxylin

Monfils, Paul PMonfils <@t> Lifespan.org
Wed Mar 15 11:46:17 CST 2006

Mayer's Hematoxylin can be purchased from Rowley Biochemical Institute, as
can a wide range of dyes (both dry powders and readymade solutions), which
in fact is the only thing they sell.  I have used their products for years
and have rarely run into any problems.  When I did have one problem with a
lot of orcein, they promptly dealt with it, sending me samples of other lots
at no charge until I found one that worked to my satisfaction.


> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Bauer, Karen
> Sent: 	Wednesday, March 15, 2006 9:02 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Mayer's Hematoxylin
> Hello to all,
> I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our
> routine H&E staining procedure.  We make up our Mayer's Hematoxylin from
> scratch, but have recently been given permission to buy something that is
> pre-made.  We've tried Harris, but the doctors do not like the
> differentiation that take place with using that kind of hematoxylin.  (We
> stain by hand, so one tech's dip might be longer or shorter than another
> tech's dip in the differentiator.)  They are seeing variations in nuclear
> staining, no matter how careful we are with our "dips".  
> I'm looking for something that stains tissues in 5-10 minutes and does not
> need differentiation.  Everything that I've tried so far is not working
> out to their satisfaction.  We use a Gill's III for our FS set up, and
> they do not want that either (for the routine H&E's).
> Does anyone have any ideas or tips for me?  I'll be greatly appreciative.
> Thanks in advance,
> Karen Bauer HT(ASCP)
> Histology Supervisor
> Luther Hospital
> Eau Claire,  WI
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