[Histonet] Ki-67 on skin tissue

[Mildred Fail]

We did get that clone to work but not as well or as consistently as Ki-67 clone K3 from Cell Marque. The dilution 1:50 with HIER in EDTA

Rena Fail

>>> "Coolen, Neeltje" <ncoolen <@t> burns.nl> 03/03/06 09:27AM >>>
Dear Histonetters,
 
I am trying to get Ki-67 clone Ki-S5 (labVision/Neomarkers) working on
FFPE skin tissue. I have tried HIER in citrate buffer pH 6.0 and in
Tris/EDTA buffer pH 9.0, but this does not work.
Does anyone have an idea?
 
Thank you.
 
Neeltje Coolen
 
--------------------------------------
N.A. Coolen, PhD student
VSBN
Zeestraat 29
1941 AJ Beverwijk
The Netherlands
 
tel: +31 251 275 564
 
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