[Histonet] Bone Marrow Decaling for IHC

[Elizabeth Chlipala]

Sandy

We don't do a lot of bone marrow cores, but we run a lot of immunos on
rat, mouse and goat joints.  I find that formic acid works well, we use
a concentration of 5 to 10%.  For antigen retreival I like to use
proteinase K or other enzymatic methods.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sandosis <@t> uia.net
Sent: Wednesday, March 01, 2006 2:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: mark.lones <@t> stjoe.edu
Subject: [Histonet] Bone Marrow Decaling for IHC


Hello,

Does anyone have sugestions for a "gentle" yet "effective" method of 
decaling bone marrow cores so that the antigenic sites remain viable?

Thank you,
Sandy

PS Thanks for all the response on clearing colon fat to reveal lymph 
nodes, we are going to try the PenFix first as we are already set for 
the haz. waste disposal for that reagent. 


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