[Histonet] Bone Marrow Decaling for IHC
Elizabeth Chlipala
liz <@t> premierlab.com
Wed Mar 1 16:24:59 CST 2006
Sandy
We don't do a lot of bone marrow cores, but we run a lot of immunos on
rat, mouse and goat joints. I find that formic acid works well, we use
a concentration of 5 to 10%. For antigen retreival I like to use
proteinase K or other enzymatic methods.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sandosis <@t> uia.net
Sent: Wednesday, March 01, 2006 2:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: mark.lones <@t> stjoe.edu
Subject: [Histonet] Bone Marrow Decaling for IHC
Hello,
Does anyone have sugestions for a "gentle" yet "effective" method of
decaling bone marrow cores so that the antigenic sites remain viable?
Thank you,
Sandy
PS Thanks for all the response on clearing colon fat to reveal lymph
nodes, we are going to try the PenFix first as we are already set for
the haz. waste disposal for that reagent.
---------------------------------------------
This message was sent using the UIA Web Mail Server.
ULTIMATE Internet Access, Inc http://www.uia.net/
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
__________ NOD32 1.1423 (20060301) Information __________
This message was checked by NOD32 antivirus system. http://www.eset.com
More information about the Histonet
mailing list