[Histonet] Staining MMA flourochrome labeled sections

[Casey Holliday]

Hello all,

I have a modest number of bone-labeled specimens about ready to be
"fixed" and embedded in MMA. To make the most out of the data set, I
was hoping to stain (Toluidine Blue) the same sections for tissue
morphology that I was going to use for studying bone deposition. Are
there any problems, hinderances, or special techniques with using
T-Blue (or another preferential stain) on these tetracycline/calcein
labeled slides? Second, is alcoholic formalin now considered to be
superior to alcohol for retaining the tissue's morphology in plastic
embedding/sectioning.

Many thanks

Casey

-- 
Casey Holliday, Ph.D.
Instructor of Anatomical Sciences
Postdoctoral Researcher
Ohio University College of Osteopathic Medicine
Dept. Biomedical Sciences
Ohio University