[Histonet] Embedding Work Sheets
Kopczynski, Charlotte
Charlotte.Kopczynski <@t> baycare.org
Wed Jun 28 13:10:12 CDT 2006
We currently use Cerner Millennium and after the cases have been
accessioned, we print a Grossing Worklist which has several lines between
cases. This is used at the Grossing Bench where Block/pieces and any
special instructions are written manually. Then the Processing tasks are
entered into Cerner which has a field for pieces for each processing task
(H&E Block). After all the grossing info has been entered, an embedding
worklist is printed along with slide labels. The person embedding, checks
the worklist and initials each case. If any discrepancies occur, the
embedder follows up with the Pathologist or PA. When the slides are
stained, coverslipped and labeled, the blocks are checked against the slides
and the worklist is initialed before delivery to the pathologist.
Thanks,
Charlotte Kopczynski,HTL(ASCP)
Baycare Pathology Manager
Phone: 727-461-8246
Fax: 727-462-7597
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, June 28, 2006 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 31, Issue 40
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: Chromogens that survive HIER (Jacqui Detmar)
2. Any suggestion on Promega or Chemicon TUNEL System on human
tissue. (soofia siddiqui)
3. lipofuscin detection (Sally Mak)
4. who grosses and embeds? (RSRICHMOND <@t> aol.com)
5. RE: who grosses and embeds? (Weems, Joyce)
6. IHC Workshop in California (bjdewe <@t> aol.com)
7. Re: who grosses and embeds? (Rene J Buesa)
8. RE: Air purifier (Osborn, Sharon)
9. RE: who grosses and embeds? (Kemlo Rogerson)
10. Re: who grosses and embeds? (Joe Nocito)
11. RE: who grosses and embeds?
(Marshall Terry Dr, Consultant Histopathologist)
12. CD68 in FFPE mouse tissue (Einar J?rundsson)
13. Anti Glycine Transporter 1 (Susan.Ferrigon <@t> sanofi-aventis.com)
14. Dako Autostainer (Lester Raff)
15. Re: Dako Autostainer (Rene J Buesa)
16. Davidson's (Webb, Dorothy L)
17. RE: Any suggestion on Promega or Chemicon TUNEL System
onhuman tissue.. . (Henry, Charlene)
18. Re: who grosses and embeds? (RSRICHMOND <@t> aol.com)
19. need safety manual info (Ron Martin)
20. RE: Dako Autostainer (Tom McNemar)
21. TBS Microwave Programs (themagoos <@t> rushmore.com)
----------------------------------------------------------------------
Message: 1
Date: Tue, 27 Jun 2006 13:31:37 -0400
From: "Jacqui Detmar" <detmar <@t> mshri.on.ca>
Subject: RE: [Histonet] Chromogens that survive HIER
To: "Rachael Emerson" <rachael_emerson <@t> urmc.rochester.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A249C197854D3442BDAE4C6BA4D5553C3F9F0B <@t> ex1.ad.mshri.on.ca>
Content-Type: text/plain; charset="iso-8859-1"
Hi there. I have done HIER (citrate buffer, pH 6) on DAB-stained mouse
placentae and it works great.
Jacqui Detmar
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rachael
Emerson
Sent: Monday, June 26, 2006 3:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Chromogens that survive HIER
Hello. I am working on a double stain, in which my first round of developing
I use Vector Blue.
I then do a HIER and proceed....
The Vector Blue stains beautifully and it remains the same even after going
through heat induced antigen retrieval.
Can anyone tell me if there are other visualizations systems, besides Vector
Blue, that can withstand going through HIER?
Thank you
Rachael Emerson
--
Rachael L. Emerson
Center for Pediatric Biomedical Research
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Tue, 27 Jun 2006 10:55:26 -0700 (PDT)
From: soofia siddiqui <soofias2 <@t> yahoo.com>
Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System
on human tissue.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060627175526.25340.qmail <@t> web39504.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I am going to start Staining on human skin Using TUNEL System. I wanted to
get some information about the vender if someone have succesfully used any
system on human tissue.I am looking at Promega and Chemicon but really want
to get some feed back if someone have used any of them or some other .
ss
---------------------------------
Yahoo! Sports Fantasy Football '06 - Go with the leader. Start your league
today!
------------------------------
Message: 3
Date: Tue, 27 Jun 2006 10:57:35 -0700 (PDT)
From: Sally Mak <skmak1886 <@t> yahoo.com>
Subject: [Histonet] lipofuscin detection
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060627175735.97203.qmail <@t> web53305.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I would like to know how to detect the lipofuscin in the frozen fixed brain
tissue. I tried carbol fuchsin solution but it seems not so good at all.
Thanks for sharing.
---------------------------------
Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates.
------------------------------
Message: 4
Date: Tue, 27 Jun 2006 14:46:05 EDT
From: RSRICHMOND <@t> aol.com
Subject: [Histonet] who grosses and embeds?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4ff.1ccebd8.31d2d6ed <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
The question of who is allowed to gross what surgical pathology specimens
seems to keep coming up. Right now the regulatory agencies seem to be
leaning
toward making the pathologist do it all.
In contrast, nobody seems to be interested in the embedder - who in effect
re-grosses small biopsy specimens. When I was a resident (around 1970) we
used
to fill out a sheet - listing the number of biopsy specimens for example -
that
the embedder consulted while embedding. I've worked in maybe forty surgical
pathology services since then (as a locum tenens pathologist, mostly) and I
don't think I've ever seen an embedding sheet again.
It's quite disconcerting when the endoscopist describes three specimens, I
receive two, and the next day there's only one piece of tissue on the slide.
It seems to me that the College of American Pathologists (CAP) should make
it
a Phase II (immediate remediation required) to embed small specimens without
an embedding sheet.
Cowpath, Tantrum, and other surgical pathology data bases should be able to
generate a compact case list that the count of specimens could be
handwritten
onto - Cowpath at least requires that a compact specimen log be handwritten.
And pathology residents should be required to come in during the wee hours
and learn how to embed!
Bob Richmond
Knoxville TN and Gastonia NC
------------------------------
Message: 5
Date: Tue, 27 Jun 2006 14:54:27 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] who grosses and embeds?
To: <RSRICHMOND <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1CD6831EB9B26D45B0A3EAA79F7EBD3202960104 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="utf-8"
Well said. We would pass on that requirement as we do still count pieces -
written on the requisition for the transcriptionist to put in the gross
template, and transferred to the Triple G system for the embedders. If we
don't have the correct number of pieces, it is brought to the attention of
the pathologist.
Joyce
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
RSRICHMOND <@t> aol.com
Sent: Tuesday, June 27, 2006 2:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] who grosses and embeds?
The question of who is allowed to gross what surgical pathology specimens
seems to keep coming up. Right now the regulatory agencies seem to be
leaning
toward making the pathologist do it all.
In contrast, nobody seems to be interested in the embedder - who in effect
re-grosses small biopsy specimens. When I was a resident (around 1970) we
used
to fill out a sheet - listing the number of biopsy specimens for example -
that
the embedder consulted while embedding. I've worked in maybe forty surgical
pathology services since then (as a locum tenens pathologist, mostly) and I
don't think I've ever seen an embedding sheet again.
It's quite disconcerting when the endoscopist describes three specimens, I
receive two, and the next day there's only one piece of tissue on the slide.
It seems to me that the College of American Pathologists (CAP) should make
it
a Phase II (immediate remediation required) to embed small specimens without
an embedding sheet.
Cowpath, Tantrum, and other surgical pathology data bases should be able to
generate a compact case list that the count of specimens could be
handwritten
onto - Cowpath at least requires that a compact specimen log be handwritten.
And pathology residents should be required to come in during the wee hours
and learn how to embed!
Bob Richmond
Knoxville TN and Gastonia NC
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the
addressee listed above. If you are neither the intended recipient nor the
employee or agent responsible for delivering this message to the intended
recipient, you are hereby notified that any disclosure, copying,
distribution or the taking of any action in reliance on the contents of this
information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer. Thank you. Saint Joseph's Health System,
Inc.
------------------------------
Message: 6
Date: Tue, 27 Jun 2006 15:09:48 -0400
From: bjdewe <@t> aol.com
Subject: [Histonet] IHC Workshop in California
To: Histonet <@t> lists.utsouthwestern.edu,
MICROSCOPY <@t> LISTSERV.MED.HARVARD.EDU
Message-ID: <8C868346502B3F2-1BB0-DB7 <@t> mblk-d36.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
We still have a few spots left for our IHC on Animal Tissue workshop at UC
Davis on July 28th. Here is a link to our flyer.
http://www.vetmed.ucdavis.edu/vsr/dil/events.html
To register go to:
www.innvx.com and click on workshop on the home page...
If you have any questions please ask;-)
Cheers,
Loralei Dewe
Live as if you were to die tomorrow.
Learn as if you were to live forever. - Gandhi -
*******************************************************************
Confidentiality Notice: This e-mail message, including any attachments, is
for the sole use of the intended recipient(s)and may contain confidential
and privileged information. Any unauthorized review, use, disclosure or
distribution is prohibited.
If you are not the intended recipient, please contact the sender by reply
e-mail and destroy all copies of the original message.
*******************************************************************
________________________________________________________________________
Check out AOL.com today. Breaking news, video search, pictures, email and
IM. All on demand. Always Free.
------------------------------
Message: 7
Date: Tue, 27 Jun 2006 12:51:41 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] who grosses and embeds?
To: RSRICHMOND <@t> aol.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060627195141.6616.qmail <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The number of biopsy pieces in a log form to be consulted by the embedding
HT is standard proceure at Mount Sinai Medical Center (MIami Beach), Ohio
State University and Quest Diagnostics (South Florida).
René J.
RSRICHMOND <@t> aol.com wrote:
The question of who is allowed to gross what surgical pathology specimens
seems to keep coming up. Right now the regulatory agencies seem to be
leaning
toward making the pathologist do it all.
In contrast, nobody seems to be interested in the embedder - who in effect
re-grosses small biopsy specimens. When I was a resident (around 1970) we
used
to fill out a sheet - listing the number of biopsy specimens for example -
that
the embedder consulted while embedding. I've worked in maybe forty surgical
pathology services since then (as a locum tenens pathologist, mostly) and I
don't think I've ever seen an embedding sheet again.
It's quite disconcerting when the endoscopist describes three specimens, I
receive two, and the next day there's only one piece of tissue on the slide.
It seems to me that the College of American Pathologists (CAP) should make
it
a Phase II (immediate remediation required) to embed small specimens without
an embedding sheet.
Cowpath, Tantrum, and other surgical pathology data bases should be able to
generate a compact case list that the count of specimens could be
handwritten
onto - Cowpath at least requires that a compact specimen log be handwritten.
And pathology residents should be required to come in during the wee hours
and learn how to embed!
Bob Richmond
Knoxville TN and Gastonia NC
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+
countries) for 2¢/min or less.
------------------------------
Message: 8
Date: Tue, 27 Jun 2006 17:55:25 -0400
From: "Osborn, Sharon" <SHARON.OSBORN <@t> SPCORP.COM>
Subject: [Histonet] RE: Air purifier
To: <histonet <@t> lists.utsouthwestern.edu>, <histosci <@t> shentel.net>
Message-ID:
<9A919A5D70313A4D9C56A025710874080C731D <@t> kenmsg40.us.schp.com>
Content-Type: text/plain; charset="us-ascii"
Tom,
For over two years now, we have had "R2D2" sitting on the floor of
our lab so it can circulate the air while removing the wonderful
perfumes that histology is well known for. The comments/complaints have
decreased so much that other departments are clamoring for our $1500.00
solution. Additional filters are $449 each. We got this through
ml-MarketLab JL9390 for the Air Filtration System with Filter.
Replacement Filter is JL9266.
www.MarketLabInc.com or 1.800.237.3604. Now, we very rarely have a
complaint from someone about the odors; so that says it is working well.
We leave it on 24/7 at the medium setting. The high setting stirs up
too much dust for me allergies.
sharon osborn
DNAX, SP BioPharma
Palo Alto, CA
Date: Tue, 27 Jun 2006 09:44:44 -0400
From: "HSRL" <histosci <@t> shentel.net>
Subject: [Histonet] Air Purifier for Xylene
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <001201c699ef$dc457210$0500a8c0 <@t> HSRLMAIN>
Content-Type: text/plain; charset="us-ascii"
Dear Netters,
Has anyone used an air purifier made specifically for formaldehyde and
xylene fumes? I know Newcomer Supply has started distributing the
system. Does anyone have any feedback on the Newcomer system or any
other air purifying system?
Thanks for your feedback,
Tom Galati
Laboratory Director
HSRL, Inc.- A GLP Compliant Contract Laboratory
5930 Main Street
Mount Jackson, Virginia 22842
540.477.4440
Fax: 540.477.4448
tomgalati <@t> hsrl.org
www.hsrl.org
*********************************************************************
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information
included in this message is prohibited -- Please
immediately and permanently delete.
------------------------------
Message: 9
Date: Wed, 28 Jun 2006 09:30:26 +0100
From: Kemlo Rogerson <kemlo.rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] who grosses and embeds?
To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, RSRICHMOND <@t> aol.com,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B4FA3DD12D42DA11A5DA00508BAF864902FE014D <@t> WAHTNTEX2.waht.swest.nhs.uk>
Content-Type: text/plain
IMHO those who gross and embed should be those with the competency and
training to do it; it could be a Pathologists, he/she could delegate to a SR
or 'Junior Medic' that they have trained or it could be delegated to a BMS
(Histotech) who is qualified (Specialist Exam) and competent.
I have personally grossed skin biopsies, uterus, dead foals and budgies.
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
People are like stained glass windows: they sparkle and shine when the sun
is out, but when the darkness sets in their true beauty is revealed only if
there is a light within. --Elizabeth Kubler-Ross
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your
co-operation
------------------------------
Message: 10
Date: Wed, 28 Jun 2006 05:47:46 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] who grosses and embeds?
To: <RSRICHMOND <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006001c69aa0$4b1d15c0$0b69ce44 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Dr. R,
you bring up an excellent point. We have one pathologist that refuses to cut
shave bxs, vas deferens, fallopian tubes, temporal arteries and other small
specimens. I have instructed my embedders that they have to cut these
specimens for proper orientation. Is this considered grossing?
As far as grossing sheets, every lab that I have worked in, plus the lab
that I started, always had a grossing sheet. It's almost mal-practice not
to. How could you tell if a specimen has special instructions or not such as
a renal bx?
Just my 3 cents worth.
Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: <RSRICHMOND <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, June 27, 2006 1:46 PM
Subject: [Histonet] who grosses and embeds?
> The question of who is allowed to gross what surgical pathology specimens
> seems to keep coming up. Right now the regulatory agencies seem to be
> leaning
> toward making the pathologist do it all.
>
> In contrast, nobody seems to be interested in the embedder - who in effect
> re-grosses small biopsy specimens. When I was a resident (around 1970) we
> used
> to fill out a sheet - listing the number of biopsy specimens for example -
> that
> the embedder consulted while embedding. I've worked in maybe forty
> surgical
> pathology services since then (as a locum tenens pathologist, mostly) and
> I
> don't think I've ever seen an embedding sheet again.
>
> It's quite disconcerting when the endoscopist describes three specimens, I
> receive two, and the next day there's only one piece of tissue on the
> slide.
>
> It seems to me that the College of American Pathologists (CAP) should make
> it
> a Phase II (immediate remediation required) to embed small specimens
> without
> an embedding sheet.
>
> Cowpath, Tantrum, and other surgical pathology data bases should be able
> to
> generate a compact case list that the count of specimens could be
> handwritten
> onto - Cowpath at least requires that a compact specimen log be
> handwritten.
>
> And pathology residents should be required to come in during the wee hours
> and learn how to embed!
>
> Bob Richmond
> Knoxville TN and Gastonia NC
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> --
> No virus found in this incoming message.
> Checked by AVG Free Edition.
> Version: 7.1.394 / Virus Database: 268.9.5/376 - Release Date: 6/26/2006
>
>
------------------------------
Message: 11
Date: Wed, 28 Jun 2006 12:19:08 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] who grosses and embeds?
To: "Kemlo Rogerson" <kemlo.rogerson <@t> waht.swest.nhs.uk>, "Rene J
Buesa" <rjbuesa <@t> yahoo.com>, <RSRICHMOND <@t> aol.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F05F54A7C <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
So, you got a feather in your cap after flogging a dead horse.
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Kemlo Rogerson [mailto:kemlo.rogerson <@t> waht.swest.nhs.uk]
Sent: 28 June 2006 09:30
To: 'Rene J Buesa'; RSRICHMOND <@t> aol.com;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] who grosses and embeds?
IMHO those who gross and embed should be those with the competency and
training to do it; it could be a Pathologists, he/she could delegate to a SR
or 'Junior Medic' that they have trained or it could be delegated to a BMS
(Histotech) who is qualified (Specialist Exam) and competent.
I have personally grossed skin biopsies, uterus, dead foals and budgies.
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
People are like stained glass windows: they sparkle and shine when the sun
is out, but when the darkness sets in their true beauty is revealed only if
there is a light within. --Elizabeth Kubler-Ross
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your
co-operation
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 28 Jun 2006 12:49:22 +0000
From: Einar J?rundsson <einar.jorundsson <@t> decode.is>
Subject: [Histonet] CD68 in FFPE mouse tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF453DAAEE.4D6241BC-ON0025719B.0045AF44-0025719B.0046704F <@t> decode.is>
Content-Type: text/plain; charset="US-ASCII"
I am having problems with IHC staining with macrophage-markers in
formalin-fixed paraffin-embedded (FFPE) mouse arotic root sections. F4/80
(Serotec) works well in spleen (after proteinase K digestion) but is
inconsistent in aortas. I have also tried CD68 clone FA11 (Serotec) with
proteinase K digestion and HIER (citrate, borate, Tris-HCl) with no
success. Any tips?
Regards, Einar.
------------------------------
Message: 13
Date: Wed, 28 Jun 2006 14:00:28 +0100
From: <Susan.Ferrigon <@t> sanofi-aventis.com>
Subject: [Histonet] Anti Glycine Transporter 1
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<90B6684A9D6DAF468F7A5DC148754E1D2154D4 <@t> ALPW31.f2.enterprise>
Content-Type: text/plain; charset="iso-8859-1"
Hi Does anyone have any experience/Protocols for ICC staining with
Anti-Glycine Transporter 1 on rat Brain/Liver
This is a new one for me and have been asked if I can try to get a method
working pretty quickly, so if anyone has any experience they could share I
would be really grateful.
Thanks
Susan
------------------------------
Message: 14
Date: Wed, 28 Jun 2006 08:34:03 -0500
From: "Lester Raff" <LRaff <@t> lab.uropartners.com>
Subject: [Histonet] Dako Autostainer
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5DA1CA5D0B98A84985B545A24423B822A501 <@t> UPLAB01.uplab.local>
Content-Type: text/plain; charset="iso-8859-1"
Hello, and thanks to all for their input on the PASCAL pressure cooker.
Another IHC question. We use the Dako Autostainer Plus and occassionaly
have areas on the slides that don't seem to stain. We are careful not to
let the slides dry out. Do Autostainers have a problem distributing
reagents evenly?
Thanks,
Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph: 708-486-0076
fax: 708-486-0080
------------------------------
Message: 15
Date: Wed, 28 Jun 2006 06:48:52 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Dako Autostainer
To: Lester Raff <LRaff <@t> lab.uropartners.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060628134852.21343.qmail <@t> web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
They should not but if you are diluting concentrated Abs you should remember
to add Tween 20 to assure even distribution.
René J.
Lester Raff <LRaff <@t> lab.uropartners.com> wrote:
Hello, and thanks to all for their input on the PASCAL pressure cooker.
Another IHC question. We use the Dako Autostainer Plus and occassionaly have
areas on the slides that don't seem to stain. We are careful not to let the
slides dry out. Do Autostainers have a problem distributing reagents evenly?
Thanks,
Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph: 708-486-0076
fax: 708-486-0080
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call
rates.
------------------------------
Message: 16
Date: Wed, 28 Jun 2006 09:43:06 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Davidson's
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA270171FE69 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
Has anyone had any problems with Davidson's fixative interfering with
their IHC staining? We have not run any comparison studies, but, one of
our pathologists heard "whispers" of this amongst fellow pathologists at
another facility!! Any thoughts on this would be appreciated!!
------------------------------
Message: 17
Date: Wed, 28 Jun 2006 10:38:22 -0500
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
Subject: RE: [Histonet] Any suggestion on Promega or Chemicon TUNEL
System onhuman tissue.. .
To: "soofia siddiqui" <soofias2 <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5CB39BCA5724F349BCB748675C6CA1A2099A1CFE <@t> SJMEMXMB02.stjude.sjcrh.local>
Content-Type: text/plain; charset="US-ASCII"
We use the Promega Dead End kit and it works great; however when we do a
TUNEL assay, we also run pHistone IHC.
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of soofia
siddiqui
Sent: Tuesday, June 27, 2006 12:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System
onhuman tissue.. .
I am going to start Staining on human skin Using TUNEL System. I wanted
to get some information about the vender if someone have succesfully
used any system on human tissue.I am looking at Promega and Chemicon but
really want to get some feed back if someone have used any of them or
some other .
ss
---------------------------------
Yahoo! Sports Fantasy Football '06 - Go with the leader. Start your
league today!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Wed, 28 Jun 2006 11:46:07 EDT
From: RSRICHMOND <@t> aol.com
Subject: Re: [Histonet] who grosses and embeds?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <53c.19c47aa.31d3fe3f <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Kemlo Rogerson notes:
>>I have personally grossed skin biopsies, uterus, dead foals and budgies.<<
Presumably not John Lennon's budgie -
I have a little budgie
he is my very pal
I take him walks in Britain
I hope I always shall.
Bob Richmond
------------------------------
Message: 19
Date: Wed, 28 Jun 2006 12:04:27 -0400
From: "Ron Martin" <Pathrm35 <@t> adelphia.net>
Subject: [Histonet] need safety manual info
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000401c69acc$88789e90$2ea2ac45 <@t> D6WRV2B1>
Content-Type: text/plain; charset="US-ASCII"
If anyone is willing to share some info about their safety manual, I would
appreciate it.
I need info on: chemical hygiene plans, ergonomics and OSHA regulations.
Thanks in advance
Ron Martin
------------------------------
Message: 20
Date: Wed, 28 Jun 2006 12:33:32 -0400
From: "Tom McNemar" <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Dako Autostainer
To: "Lester Raff" <LRaff <@t> lab.uropartners.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<51D5D78FBEDAEA4FBCCD9A9D44211DC528F3D8 <@t> lmhsmail.lmhealth.org>
Content-Type: text/plain; charset="iso-8859-1"
What counterstain do you use? Hematoxylin will clumb and clog the probe
causing uneven staining.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Lester
Raff
Sent: Wednesday, June 28, 2006 9:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dako Autostainer
Hello, and thanks to all for their input on the PASCAL pressure cooker.
Another IHC question. We use the Dako Autostainer Plus and occassionaly
have areas on the slides that don't seem to stain. We are careful not to
let the slides dry out. Do Autostainers have a problem distributing
reagents evenly?
Thanks,
Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph: 708-486-0076
fax: 708-486-0080
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Wed, 28 Jun 2006 10:22:18 -0600
From: themagoos <@t> rushmore.com
Subject: [Histonet] TBS Microwave Programs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1151511738.44a2acba352de <@t> webmail.rushmore.com>
Content-Type: text/plain; charset=ISO-8859-1
We have a TBS Shurwave 1200 and we are trying to process fatty skin
specimens.
Does anybody have a sample program for microwave processing fatty skin
specimens? We are also looking at microwave processing all types of
specimens.
Any feedback would be appreciated. Thank you.
Jason McGough HT(ASCP)
Clinical LAboratory of the Black Hills
2805 5th Street
Rapid City, SD 57701
605-343-2267
-------------------------------------------------
This mail sent by http://webmail.rushmore.com
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 31, Issue 40
****************************************
Confidential: This electronic message and all contents contain information
from BayCare Health System which may be privileged, confidential or otherwise
protected from disclosure. The information is intended to be for the addressee
only. If you are not the addressee, any disclosure, copy, distribution or use
of the contents of this message is prohibited. If you have received this
electronic message in error, please notify the sender and destroy the original
message and all copies.
More information about the Histonet
mailing list