[Histonet] Chromogens that survive HIER

[Katri Tuomala]

DAB would probably survive heat retrieval, but I don't have personal 
experience to that fact. Try it.

Katri

Katri Tuomala
Hamilton, Ontario, Canada

----- Original Message ----- 
From: "Rachael Emerson" <rachael_emerson <@t> urmc.rochester.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 26, 2006 3:06 PM
Subject: [Histonet] Chromogens that survive HIER


> Hello. I am working on a double stain, in which my first round of 
> developing
> I use Vector Blue.
> I then do a HIER and proceed....
> The Vector Blue stains beautifully and it remains the same even after 
> going
> through heat induced antigen retrieval.
> Can anyone tell me if there are other visualizations systems, besides 
> Vector
> Blue, that can withstand going through HIER?
>
> Thank you
> Rachael Emerson
>
> -- 
> Rachael L. Emerson
> Center for Pediatric Biomedical Research
> University of Rochester Medical Center
> 575 Elmwood Avenue MRBX 1.11301
> Rochester, NY 14642
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet