[Histonet] Chromogens that survive HIER
katri <@t> cogeco.ca
Mon Jun 26 21:56:49 CDT 2006
DAB would probably survive heat retrieval, but I don't have personal
experience to that fact. Try it.
Hamilton, Ontario, Canada
----- Original Message -----
From: "Rachael Emerson" <rachael_emerson <@t> urmc.rochester.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 26, 2006 3:06 PM
Subject: [Histonet] Chromogens that survive HIER
> Hello. I am working on a double stain, in which my first round of
> I use Vector Blue.
> I then do a HIER and proceed....
> The Vector Blue stains beautifully and it remains the same even after
> through heat induced antigen retrieval.
> Can anyone tell me if there are other visualizations systems, besides
> Blue, that can withstand going through HIER?
> Thank you
> Rachael Emerson
> Rachael L. Emerson
> Center for Pediatric Biomedical Research
> University of Rochester Medical Center
> 575 Elmwood Avenue MRBX 1.11301
> Rochester, NY 14642
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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