[Histonet] Fixative studies

Gayle Callis gcallis <@t> montana.edu
Mon Jun 26 11:13:54 CDT 2006

Thanks to Barry for this wonderful list.

One reference, #4 Beckstead is actually about a formalin-free fixative, 
Zinc Tris Buffer  with paraffin embedded tissues when CD markers are 
compromised by formalin or PFA fixation.  Beckstead did this study for 
human CD markers in lymphomas.

At 02:03 PM 6/23/2006, you wrote:
>Some suggestions.
>This is a short list.
>1.      Mann, Gustav 1902. Physiological Histology. Methods and Theory.
>Oxford at the Clarendon Press. This text deals in detail with methods in
>histology and for fixation with the history and rationale behind using
>various chemical agents as fixatives.
>2.      Pearse A.G.E. The Chemistry and practice of Fixation. Chapter 5
>pages 97 -158. In Histochemistry Theoretical and Applied vol 1. 4th
>edition. Preparative and Optical Techniques. 1980. Excellent description
>of fixation chemistry and extensive list of references. vol. 2.
>Analytical techniques 1985. vol 3. Enzyme Histochemistry 1991. Churchill
>Livingstone, London.
>3.      Arnold M.M., Srivastava S., Fredenburgh J., Stockyard C.R. and
>Myers R.B. 1996. Effects of fixation and tissue processing on
>immunohistochemical demonstration of specific antigens. Biotechnology &
>Histochemistry 71: 224-230.
>4.      Beckstead J.H. 1994. A Simple Technique for Preservation of
>Fixative-sensitive Antigens in Paraffin-embedded Tissues: J. Histochem.
>Cytochem. 43: 345.
>5.      Isaam Eltoum, Jerry Fredenburgh, Russel B. Myers and William
>Grizzle. 2001.  Introduction to the theory and Practice of Fixation of
>Tissues. J. Histotechnology 24 #3 173- 190.
>6.      Hopwood D. 1985. Cell and tissue fixation, 1972 - 1982.
>Histochemical Journal. 17: 389-442
>7.      Prento P and Lyon H. 1997. Commercial Formalin Substitutes for
>Histopathology. Biotechnic & Histochemistry. 72 (5). 272-282.

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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