[Histonet] Sirius Red Protocol mouse pancreas
JEFF TATUM ZELIADT
jzeliadt <@t> msn.com
Thu Jun 15 23:53:19 CDT 2006
you are fuckin pissing me off with all these messages. pull you head
out of your ass and remove me from your lists
From: Histology SLU <sluhisto <@t> yahoo.com>
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
CC: tetriba <@t> slu.edu
Subject: [Histonet] Sirius Red Protocol mouse pancreas
Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT)
> I have a dilemma that I hope that someone out there can shed
some light on. We have an investigator that has been working with
mouse FFPE pancreas. We have been doing the processing, embedding,
sectioning, and sirius red staining. Lately, however, we have been
getting too much red, more than the collagen is staining. The
staining pattern is everywhere!! This is a new development and we
have tried the following:
> 1. Making new reagents
> 2. Evaluating the processes that the mouse went through prior
> 3. Did a fixation evaluation to make sure that fixation was
not an issue.
> 4. Multiple techs performing stain
> We are at a loss to help this investigator and don't want to
give up!! We had performed this stain numerous times in the past
for this investigator without any problems.
> Any help you can send my way would be appreciated by myself as
well as the investigator.
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