[Histonet] Glucose Oxidase blocking for peroxidase, glucose source
JEFF TATUM ZELIADT
jzeliadt <@t> msn.com
Thu Jun 15 23:52:18 CDT 2006
remove my email from you damn lists fuck head
______________________________________________________________
From: Gayle Callis <gcallis <@t> montana.edu>
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Glucose Oxidase blocking for peroxidase,
glucose source
Date: Thu, 15 Jun 2006 11:34:51 -0600
>To all using Glucose oxidase endogenous peroxidase blocking, be
>aware that Sigma has discontinued the beta-D(+) glucose used for
>this blocking. Some have tried another glucose as a substitute
and
>the peroxidase was not blocked.
>
>Since I tout this highly useful peroxidase blocking method, I
found
>a source of the glucose
>
>ICN Cat#100953 beta-D Glucose. this contain up to 10% of
>alpha-isomer, although Sigma's contained only 4%.
>
>Hopefully this will not be discontinued as Sigma has done. If
there
>are any doubts, call Sigma Technical Services and ask them for a
>source of this glucose.
>
>Never thought there would be so much difference in a glucose
>molecule
>
>
>
>
>
>
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
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