[Histonet] Glucose Oxidase blocking for peroxidase, glucose source

[Gayle Callis]

To all using Glucose oxidase endogenous peroxidase blocking, be aware that 
Sigma has discontinued the beta-D(+) glucose used for this blocking.  Some 
have tried another glucose as a substitute and the peroxidase was not 
blocked.

Since I tout this highly useful peroxidase blocking method, I found a 
source of the glucose

ICN Cat#100953 beta-D Glucose.  this contain up to 10% of alpha-isomer, 
although Sigma's contained only 4%.

Hopefully this will not be discontinued as Sigma has done.  If there are 
any doubts, call Sigma Technical Services and ask them for a source of this 
glucose.

Never thought there would be so much difference in a glucose molecule







Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)