[Histonet] Re:Sirius Red Protocol mouse pancreas

[JEFF TATUM ZELIADT]

       ______________________________________________________________

     From:  Jamie E Erickson <jamie.erickson <@t> abbott.com>
     To:  histonet <@t> lists.utsouthwestern.edu
     Subject:  [Histonet] Re:Sirius Red Protocol mouse pancreas
     Date:  Thu, 15 Jun 2006 13:48:11 -0400
     >Susan,
     >                  We  also  do  Sirius  red  staining but of mouse
     lungs, we use
     >a  polarizing  light  to quantitative collagen deposition. We have
     not see
     >and  appreciable  increase  in  red  staining  over  time. Maximum
     staining
     >intensity  is  reach  after  90  minutes  in  the  paper by L.C.U.
     Junqueira
     >Histochemical   Journal   1979,  11,447-445.  Check  the  histonet
     achieves that's
     >where I got most of my information.
     >                  As far as the staining I would run an old sample
     to
     >determine  if  that stains differently then it had in the past, if
     it does
     >then it's a staining issue if not it's the tissue, it could be an
     >experimental  issue.  Our  investigators  usually  give us control
     samples
     >untreated  Lung  or we can ask for muscle or kidney for a positive
     control
     >I'd  look  into  the tissue being the issue if you haven't already
     done that.
     >
     >Hope that helps.
     >
     >Jamie
     >
     >_______________________________
     >Jamie Erickson
     >Sr. Research Associate
     >Department: DSMP
     >Abbott Bioresearch Center
     >100 Research Drive
     >Worcester, MA 01605-4341
     >508-688-3134
     >FAX: 508-793-4895
     >e-mail: jamie.erickson <@t> abbott.com
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