[Histonet] immuno background staining from agarose cells blocks

Elizabeth Chlipala liz <@t> premierlab.com
Thu Jun 15 11:09:08 CDT 2006


Hello All
 
I had prepared some agarose cell blocks that contained bovine embryos
and cut unstained slides for a client.  I'm not performing the immunos
the client is and they experienced alot of background from the agarose
during the stainining process.  Is there a way to get rid of the
background staining or remove the agarose without loosing the embryos.
The person that is performing the immunos follows appropriate protocols
with blocking steps.  Any help would be appreciated .
 
Thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/> 
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 
 


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