[Histonet] toe nails

[Anthony F. Boris]

We use charged slides from Premiere.  keep in 65 degree oven for 30 minutes and stain.   We have not had any fall off since we switched to these slides.    When cutting you can use a 5% solution of KOh for a "surface decal".   This breaks down the keratin a little bit (same stuff as in NAIR).   Helps to get decent sectioning.
 
Tony

	-----Original Message----- 
	From: Chris Pomajzl [mailto:cpomajzl <@t> cpllabs.com] 
	Sent: Wed 6/14/2006 10:41 AM 
	To: HISTONET 
	Cc: 
	Subject: Re: [Histonet] toe nails
	
	

	Good question. We have dealt with this issue for many years, and we have
	come up with a solution that has worked for some time.
	
	First of all, sections are picked up on "+" coated slides that have been
	smeared with egg albumin. We separate eggs from the grocery store and keep a
	stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it
	refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and
	smear to cover all of the glass.
	
	We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra
	for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20
	minutes prior to staining the following day.
	
	This seems to work very well for us.
	
	----- Original Message -----
	From: "Conlon, Paula F." <Paula.F.Conlon <@t> Lahey.org>
	To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
	Sent: Wednesday, June 14, 2006 9:16 AM
	Subject: [Histonet] toe nails
	
	
	Hi Histonetters, does anyone have a good procedure for sectioning nails and
	keeping them on the slides during h&e staining and pas staining?Most of the
	time our sections wash off. Thanks for your help.
	
	
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