[Histonet] Minimizing shrinkage

[Geoff McAuliffe]

Hi Tim:

    Any processing will induce some shrinkage so my first suggestion is 
to measure fresh or fixed tissue with a dissecting microscope. If that 
is not possible I suggest a thorough fixation in formalin or 
formalin+glutaraldehyde followed by embedding in an epoxy resin (Epon 
substitute). Much less shrinkage in epoxies as compared to wax or 
methacrylates.

Geoff

Timothy Macatee wrote:

>I have someone who wants to measure cardiac vessel diameter.  Does anyone
>have an idea of the best process to avoid shrinkage of the tissue?  Notice,
>how I made no reference to any situation comedy television shows.
>
>Tim  
>  
>


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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