[Histonet] brain tissue sections

Thu Jun 8 15:20:03 CDT 2006

Thanks everybody for the help with the rat brains falling off, I am not
having this problem with any other tissue except the brains, when I say
silane coated slides I mean plus charged slides (APES) I buy commercially
from several vendors, I was under the impression that all plus charged
slides were charged by coating with silane, so to me silane coated slides
and plus charged slides were the same thing?????  I cut sections at 4
microns and airdried for only 20 min before heating at 55dc.  The consenses
seems to be to airdry overnight before heating the next day and I am trying
that today.
Thanks again,
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

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-----Original Message-----
From: Nancy Lemke [mailto:nsnwl <@t> neuro.hfh.edu] 
Sent: Thursday, June 08, 2006 11:53 AM
To: Patsy Ruegg; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] brain tissue sections

Hi Patsy,
The problem you describe is because the sections have not dried enough.
LuAnn is exactly correct about air-drying over night.  If you don't air-dry,
the sections can look like they have exploded off of the slide as soon as
you hit the xylene.  This tip was given to me many eons ago by a technical
specialist at Miles in Elkhart, Ind.  He worked with  VIP processors and had
encountered this problem with one of his customers.  After trying
everything, they accidentally left the sections in a rack overnight and the
problem was gone!  I have blessed him many times over the years for saving
the remaining shreds of my sanity.  It does not matter if you use plain
glass, gel-coated or charged slides.  Rodent brains need the extra room
temperature drying and I even do the same with human samples because I
believe in superstitious behavior!
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Patsy Ruegg" pruegg <@t> ihctech.net
Date: Thu, 08 Jun 2006 14:04:26 -0400
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] brain tissue sections

> We are having a terrible time with our ffpe 4 micron rat brain tissue 
> sections falling off the slides, we are using silane coated slides, 
> air drying on edge on a small fan, then heating on a slide warmer flat 
> at 55dc for 1 hour to overnight.  Please help.  The sections are 
> falling off during deparaffinizing thru xylene and alcohols, haven't 
> even gotten to HIER and IHC yet.
> thanks for your help,
> Patsy
>  
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net <http://www.ihctech.net/>
>  
> 
> This email is confidential and intended solely for the use of the 
> Person(s) ('the intended recipient') to whom it was addressed. Any 
> views or opinions presented are solely those of the author. It may 
> contain information that is privileged & confidential within the 
> meaning of applicable law. Accordingly any dissemination, 
> distribution, copying, or other use of this message, or any of its 
> contents, by any person other than the intended recipient may 
> constitute a breach of civil or criminal law and is strictly 
> prohibited. If you are NOT the intended recipient please contact the
sender and dispose of this e-mail as soon as possible.
> 
>  
> _______________________________________________
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