[Histonet] brain tissue sections

[Charles Scouten]

I used silane to coat glass pipettes so proteins would not stick.  You
seem to be using it as the glue for proteins.  I would look into what
silane does and is for if I were you. It is a silicone compound that can
coat glass with a silicone layer.  Nothing sticks well to silicone.


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Thursday, June 08, 2006 1:04 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] brain tissue sections

We are having a terrible time with our ffpe 4 micron rat brain tissue
sections falling off the slides, we are using silane coated slides, air
drying on edge on a small fan, then heating on a slide warmer flat at
55dc for 1 hour to overnight.  Please help.  The sections are falling
off during deparaffinizing thru xylene and alcohols, haven't even gotten
to HIER and IHC yet.
thanks for your help,
Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

This email is confidential and intended solely for the use of the
Person(s) ('the intended recipient') to whom it was addressed. Any views
or opinions presented are solely those of the author. It may contain
information that is privileged & confidential within the meaning of
applicable law. Accordingly any dissemination, distribution, copying, or
other use of this message, or any of its contents, by any person other
than the intended recipient may constitute a breach of civil or criminal
law and is strictly prohibited. If you are NOT the intended recipient
please contact the sender and dispose of this e-mail as soon as
possible.

 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet