[Histonet] brain tissue sections

Charles Scouten cwscouten <@t> myneurolab.com
Thu Jun 8 13:38:58 CDT 2006

I used silane to coat glass pipettes so proteins would not stick.  You
seem to be using it as the glue for proteins.  I would look into what
silane does and is for if I were you. It is a silicone compound that can
coat glass with a silicone layer.  Nothing sticks well to silicone.

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Sent: Thursday, June 08, 2006 1:04 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] brain tissue sections

We are having a terrible time with our ffpe 4 micron rat brain tissue
sections falling off the slides, we are using silane coated slides, air
drying on edge on a small fan, then heating on a slide warmer flat at
55dc for 1 hour to overnight.  Please help.  The sections are falling
off during deparaffinizing thru xylene and alcohols, haven't even gotten
to HIER and IHC yet.
thanks for your help,
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 

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