[Histonet] Fwd: Re: brain tissue sections

[LuAnn Anderson]

>
>Hi Patsy,
>Try air drying overnight before the 55 dg warmer. I always air dry 
>overnight for specials and I never lose sections. Good luck.
>
>LuAnn Anderson HT(ASCP)
>Neuropathology lab
>University of Minnesota
>
>
>At 01:01 PM 6/8/2006, you wrote:
>>We are having a terrible time with our ffpe rat brain tissue 
>>sections falling off the slides, we are using silane coated slides, 
>>air drying on edge on a small fan, then heating on a slide warmer 
>>flat at 55dc for 1 hour to overnight.  Please help.  The sections 
>>are falling off during deparaffinizing thru xylene and alcohols, 
>>haven't even gotten to HIER and IHC yet.
>>thanks for your help,
>>Patsy
>>
>>Patsy Ruegg, HT(ASCP)QIHC
>>IHCtech, LLC
>>Fitzsimmons BioScience Park
>>12635 Montview Blvd. Suite 215
>>Aurora, CO 80010
>>P-720-859-4060
>>F-720-859-4110
>>wk email <mailto:pruegg <@t> ihctech.net>pruegg <@t> ihctech.net
>>web site <http://www.ihctech.net/>www.ihctech.net
>>
>>
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