[Histonet] XYL Substitutes vs IHC

[Favara, Cynthia (NIH/NIAID) [E]]

Question before comment, is it only the counterstain that is weak?

I will be interested to hear what other opinions are. I have used a
substitute for more years than I want to think about and the results are
more than adequate. I do however think that overall stains are not as
crisp and bright as with previously used more harmful chemicals. Could
be true or could be just fond memories of when I was far less busy,
stressed and a hell of a lot younger!  

Cheers to all,
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu] 
Sent: Thursday, June 08, 2006 6:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] XYL Substitutes vs IHC

Has anyone had difficulty with BVD/CWD IHC results when the
tissues/slides are processed through a xylene substitute (specifically
in this case Sub-X)?  I'm getting weak counterstaining and I'm wondering
if this could be the source of the problems; I've pretty much ruled out
mechanical or astrological causes.  Any help would be greatly
appreciated.  I only use the substitute because of air quality issues
within the lab although I still prefer xylene all around!  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87108

 

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