[Histonet] XYL Substitutes vs IHC

[Breeden, Sara]

Has anyone had difficulty with BVD/CWD IHC results when the
tissues/slides are processed through a xylene substitute (specifically
in this case Sub-X)?  I'm getting weak counterstaining and I'm wondering
if this could be the source of the problems; I've pretty much ruled out
mechanical or astrological causes.  Any help would be greatly
appreciated.  I only use the substitute because of air quality issues
within the lab although I still prefer xylene all around!  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87108