[Histonet] overnight HIER

[Morken, Tim - Labvision]

Mark, 
Some people are using this method in order to preserve delicate tissue that
is often damaged from high temperature AR. At lower temperatures delicate
tissue seems to stay on the slide better.  The proceedures I have seen
consist of intervals of 16 - 20 hours at 70C or so. 

Here is a paper:
Low-Temperature, Heat Mediated Antigen Retrieval (LTHMAR) on Archival
Lymphoid Sections.
Applied Immunohistochemistry and Molecular Morphology. 7(4):289, Decempber
1999

Tim Morken
Lab Vision - Neomarkers
www.labvision.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Tuesday, June 06, 2006 12:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] overnight HIER


Hi everyone.  In a meeting this morning someone mentionned that they had
been toold to do an overnight HIER treatment.  They had never heard of this
and wanted to know more. Is anyone doing this?  Is it simply heating the
slides at say 60C for 12-18 hours in a water bath or is there more to
it-what buffer, what temp??  Any information would be greatly appreciated.
Thanks Mark Elliott Vancouver BC


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