[Histonet] RE: Histonet Digest, Vol 31, Issue 4

Langxingpan langxingpan <@t> pantomics.com
Mon Jun 5 03:06:59 CDT 2006

Dear Paula,

You presumably mean ER/PR IHC scoring system rather than ISH. The key
element in the ER IHC scoring is the percentage of the nuclear staining.
Like many other labs, we set a "positive" ER result at a minimum of 5%
nuclear staining. The scoring includes "-" (<5%), "+" (5%~30%), "++"
(30%~80%) and "+++" (>80%). We also incorporate staining intensity as well,
but this will be recorded with reference to our positive control. Our ER
control is tissue array-based, which contains three cases of "-~+", "++" and
"+++" ER expressers. We produce these and other IHC control arrays. If you
want to have a try, I can send you some of the ER test control

Other people may use 10% of nuclear staining as the threshold for ER
positivity. However, NIH recommends that any positive nuclear ER staining is
considered as a positive result. 


Langxing Pan, M.D., Ph.D.
Pantomics, Inc.
457 Mariposa Street
San Francisco, CA 94107
Direct: 415-863-2380
Fax: 510-653-1227
langxingpan <@t> pantomics.com

Message: 9
Date: Fri, 02 Jun 2006 16:40:55 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Scoring of ER/PR and ISH
To: "Paula Wilder" <histo20 <@t> hotmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <44806A1702000077000004CD <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

We use Dr. Craig Allred's scoring system (see Mod Pathol
1998;11(2):155-168) for ER/PR.  What do you mean by "ISH"?


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Paula Wilder" <histo20 <@t> hotmail.com> 06/02/06 4:13 PM >>>
Hello Histonet!

Can anyone help me with this CAP question?  Our pathologist would like to
know how everyone scores their ER/PRs and ISH.  Any help would be greatly

Paula Wilder
St. Joseph Medical Center
Towson, MD 21204

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