[Histonet] Whole brain perfusion of mice

Charles Scouten cwscouten <@t> myneurolab.com
Fri Jun 2 09:04:28 CDT 2006

The ventricle size changes dramatically with traditional perfusion, and
some with any perfusion or with anoxia.  If measuring ventrical size is
the goal, you must work with living breathing animals.

Blood flow gets very close to every cell in the brain, and so perfusion
would get any perfused substance very close, within a mm surely, to any
cell in the brain.

Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
Sent: Friday, June 02, 2006 8:43 AM
To: Mr Andrew Tuck
Cc: Histonet List
Subject: Re: [Histonet] Whole brain perfusion of mice

Hi Andrew:

1. If you want "the opportunity to image a whole intact brain" with CT,
why fix at all? What artifacts, if any, does fixation induce? Maybe
fixation lowers contrast? Have you looked at a live mouse brain with CT?

When CT is done on human brains, good contrast is obtained without
2. Osmium does not penetrate tissue well, 1 mm deep at best so even
perfusing won't do much good.  And how do you know osmium will increase
the contrast with CT?


Mr Andrew Tuck wrote:

>I was looking to use micro CT (computer tomography X ray) to examine 
>newborn (P0) mice brains. The area we are most interested in is 
>measuring the ventricle size in these animals. However we also want to 
>look at the size and shape of other regions such as fiber tracts, 
>etc..In the past this was done by doing 50um slices, putting these on 
>slides, photographing the slices and using software to calculate the 
>ventricle size.
>Using micro CT offers the opportunity to image a whole intact brain.
>However, in the first scan we performed of a fixed mouse brain you 
>could see the shaodw outline of the brain but no internal detail. 
>Osmium tetroxide is the heavy metal of choice to produce good X ray 
>contrast, but does anyone know how you can perfuse an entire intact 
>brain with it ? I have not been able to find any protocols, or previous

>articles which have done this. Also I have read that osmium does not 
>penetrate whole tissue very well. If this is the case, is there another

>fixative that would give good X ray contrast and penetrate the tissue
>Andrew Tuck
>Research Assistant
>Queensland Centre for Schizophrenia Research School of Biomedical 
>Sciences University of Queensland Australia
>Tel: 07 3346 2368
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu

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