[Histonet] Re: Cre, eGFP, and B-Gal Antibodies

[Jimmy Lao]

Rhonda,
I feel the same way to Teri Johnson, Here is my
working lab protocol website, at least B-gal staining
there, we used Biogenesis goat-anti B gal, in some
tissue work but backgound still here.
I also want to hnow what others comments.
Good Luck!
Jimmy

http://saturn.med.nyu.edu/research/dg/joynerlab/protocols.html
--- "Johnson, Teri" <TJJ <@t> Stowers-Institute.org> wrote:

> Hi Rhonda,
> 
> I wish you nothing but luck with these! Out of the
> three, I'd say you will probably have success with
> the eGFP - it has been used successfully quite a bit
> in publications. We use Novus Biologicals rabbit
> polyclonal eGFP (new purified antibody) and we
> really like the results we're getting so far.
> 
> Regarding Cre and B-gal, we have had no luck with
> either of them. The cre antibodies have not worked
> at all (but I think should work well on cultured
> cells), and the B-gal has inconsistent staining that
> I can't mirror with x-gal staining. Between the
> histology facility and the researchers at our
> institute, I think we've tried practically every Cre
> and B-gal commercially available and we're not
> getting any usable results.
> 
> Let me know if you get some off list replies, I'm
> all ears (eyes!).
> 
> Best wishes,
> 
> Teri Johnson
> Stowers Institute for Medical Research
> Kansas City, MO 64110
> 
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