[Histonet] texas red imaging with fitc

caron fournier caron_fournier <@t> yahoo.ca
Fri Jul 28 08:47:19 CDT 2006


Just wanted to comment to Michelle that what Jim says is correct but spectral unmixing is not the only way to get around this. That is one way but is also the most expensive. The best bet is to make sure you have the right filter cubes for the dyes you are using and that they have a very steep cut off so that you can stop the long pass emission that they fitc has from exciting the texas red. 
   
  Caron


Caron Fournier, BSc, R.T.
Department of Orthopaedics,
Division of Orthopaedic Engineering Research,
U.B.C.
 

 		
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