[Histonet] pan human cell marker

[Elizabeth Chlipala]

Jason

We on occasion work on seeded construct sample that are implanted into
several species.  We use in-situ hybridization to determine whether or not
the cells are from a specific species.  For example we have used mouse DNA
satilite sequences to determine mouse cells from seeded porcine cells. In
that project we used a Mouse satellite DNA repeat sequence that only appears
in the Mouse but not the Pig genome.  There are several papers that look at
in-situ hybridization to determine human cells.  I'll attach one in an
additional e-mail.  We got our probes from GeneDetect and they were very
helpful with helping us pick out what we needed.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
jdr43 <@t> omega.med.yale.edu
Sent: Monday, July 24, 2006 4:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] pan human cell marker


Hi All-

I'm a medical student working on a mouse-human chimera system for studying
vascular grafts. Basically, am implanting grafts seeded with mixed
populations of human cells into immunodeficient mice. I am currently
explanting these grafts at different time points and trying to determine if
the cellular infiltrates are human or mouse in origin by
immunohistochemistry. Only problem is that I am having difficulty finding an
antibody that is a pan-human cell marker. If anyone knows if one exists for
immunohistochemistry or a better way of identifying human cells in these
grafts, I'd appreciate any and all advice.

Thanks so much you guys,


Jason Roh
Yale Medical Student
New Haven, CT 06510

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