Fw: [Histonet] tissue loss

[trine.gejsing <@t> leo-pharma.com]

----- Forwarded by Trine Gejsing/Ballerup/DK/LEO on 14-07-2006 07:13 -----


Maybe you are boiling the slides "too hard". If the tissue contains a lot 
of collagen, that gives problems.
Start out on full effect 900W or so. It would take like 9 min. When the 
buffer is boiling, immediatly set the effekt on 450W and cook for 25 min.
I assume the tisuue is on Frost+ glass ?

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Subject
[Histonet] tissue loss






Hi - I'm trying a citrate buffer antigen retrieval protocol.  After I 
have dewaxed the slides, I put them in citrate buffer - protocol says 
microwave on high for 25 minutes.  However, when I remove the slides all 
the tissue has slid off the slide.  Any suggestions? Melissa
-- 
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor of Large Animal Medicine
Director of Sports Medicine
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA 01536
Tel: 508-839-5395
Fax: 508-839-7922
Email: melissa.mazan <@t> tufts.edu


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