[Histonet] Re: Giemsa Stain

[gillian.2.brown <@t> gsk.com]

Hi,
Guess no one told me how hard it is as I do make my own.  We do have very 
ancient stocks so maybe I wont be able to once the components have gone.


Regards

Gill Brown






RSRICHMOND <@t> aol.com 
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07-Jul-2006 19:08
 
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[Histonet] Re: Giemsa Stain






Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using the 
powder. I also need a recipe for making light green from powder.<<

I've never - in more than 40 years - encountered anyone - including an 
intensely giemsophilic research lab 40 years ago - Wolbach's colophonium 
rosin and 
all - who made up their own Giemsa stain solution from the dry powder. 
It's 
supposed to be extremely difficult to do. Scrupulous exclusion of water is 

essential - that applies to stock bottles of the ready-made solution also.

Kemlo has given you adequate advice about Light Green SF. - And yes, 
Kemlo, 
many labs on this side of the pond have no books. American science 
education 
marches on into the new century.

Bob Richmond
Knoxville TN and Gastonia NC
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