[Histonet] Giemsa Stain

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Fri Jul 7 02:06:46 CDT 2006


With respect you have no books over there?

Giemsa; methylene blue eosinate 4g, azure B eosinate 5g, azure A eosinate 1g
and methylene blue chloride (85 to 88% dye content) 2g; keep as dry stain in
a cool dry place tightly stoppered. The solvent is an equal volume of 100%
ethyl alcohol and 98% glycerol and the dye is dissolved 800mg/ 100ml of the
mixed solvent.

Light green? What for? Light green has a molecular weight of 792.863 CI of
42095 and dissolves in water at 20g per 100 mls and in alcohol at 0.82 g per
100 mls. In Masson's stain a 2% aqueous light green in 1% acetic acid
solution is used. 

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
Pager 07659 597107
 
  

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