[Histonet] processing

[Steven Coakley]

Good afternoon,
   
  I'm working with mouse/rat muscle and trying to fine tune my processing schecdule.
  My last run of muscle were very dry.  I have a copy of the NSH Animal Processing Manual and trying to strike a balance between those listed on page 5-6.  I've noticed most of the protocols do not call for heat or P/V.  The scedule I currently use is as follows: 1-70% (hold), 1-80%, 2-95%, 3-100%, 3-xylene all for 30 minutes each and at 38C with P/V.  3 paraffins, 45 minutes each at 55C with P/V.  I'm fairly new to research and still trying to make the "mental" switch in handling the different tissue.
   
  Thanks everyone,
   
  Steve
   

		
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